Christian Wilms @ChrisWilms23@qoto.org

@kevinbolding Definitely open source miniature 2P scopes have some fundemental advantages over 1P Miniscopes, specifically depth of imaging, spatial resolution, and optical sectioning. But if you are aiming for imaging within 250um of tissue, only need single cell resolution, and have a brain structure or viral expression approach that doesn't require optical sectioning (which is usually the case) then 1P Miniscopes still have a lot of advantages. These being easier to build and use, ~50x to 100x cheaper, much larger FOV for potentially an order of magnitude more cells, higher fps, ability to be wire-free (and soon to be fully wireless 😉), smaller and lighter, and more flexible cabling when wired (potentially the most important aspect for freely behaving experiments).

It is absolutely amazing to see the growth in this area of the last decade and can't even imagine what will come out over the next.

@jonahbk @waitworry the lack of masking in medical and science fields is the most surprising to me. My own background in biology is a big part of why I feel very confident that masking is the right choice based on the current science.

I went to my optometrist this morning. They don't require customers to mask anymore, but were happy to have all their staff wear masks, including receptionists.

After my appointment finished, I noticed that all the waiting patients had masks as well, which I originally thought was just good luck. However, the receptionist told me that every customer had entered without a mask, and within a few minutes they all either put on their own mask or asked for one from the receptionist, noticing that they were the odd one out.

This experience powerfully showed me that for many, the decision to mask or not is heavily influenced by peer pressure.

Dive into the tiny universe #realtimemicroscopy

@kevinbolding Pixel throughput is much higher with 1p. More pixels + faster framerate— might be critical for fast signals like GluSnFR.

Price is low; 50k - 100k USD lower— no laser. So scaling up to multiple rigs is much more feasible.

It’s possible to go completely wireless — no tether — with 1p (not yet possible for 2p).

MINI2p photonic fiber designed for just one wavelength. 1p imaging can do multiple excitation colors.

Integration with optogenetic stimulation is easier in 1p.

Given your current salary/wage, which would you prefer:

1. 𝟰-𝗱𝗮𝘆 '𝗰𝗼𝗺𝗽𝗿𝗲𝘀𝘀𝗲𝗱" 𝘄𝗼𝗿𝗸𝘄𝗲𝗲𝗸𝘀, with 32-hour weeks and the same workload you have now.

2. 𝟴-𝘄𝗲𝗲𝗸 𝗽𝗮𝗶𝗱 𝘀𝗮𝗯𝗯𝗮𝘁𝗶𝗰𝗮𝗹 𝗮𝗳𝘁𝗲𝗿 𝗲𝘃𝗲𝗿𝘆 𝟱 𝘆𝗲𝗮𝗿𝘀 𝗼𝗳 𝘄𝗼𝗿𝗸 (sabbatical must be taken in 8-consecutive weeks — in addition to your usual PTO — to do whatever you want, but ideally with some goal related to personal or prof'l development).

[Boosts greatly appreciated! I don't have a lot of followers but would love to get at least 100 votes.]

Today was ... interesting. If you followed me for the past months over on the shitbird site, you might have seen a bunch of angry German words, lots of graphs, and the occassional news paper, radio, or TV snippet with yours truely. Let me explain.

In Austria, inflation is way above the EU average. There's no end in sight. This is especially true for basic needs like energy and food.

Our government stated in May that they'd build a food price database together with the big grocery chains. But..

Gov. Kristi Noem, the candidate of Christian family values, has been carrying on a secret extramarital affair with Trump’s ex campaign manager, Corey Lewandowski.

Rep. Lauren Boebert, who urged parents to take their kids to CHURCH not DRAG SHOWS, was caught in a public performance of Beetlejuice, a children’s show, groping and getting groped by her date.

I’ve had it with their hypocritical moralizing. They have no standing to judge or constrain ANYONE else’s private life.

This is Mastodon and this is why it rocks!

I'm giving up eating chocolate for a month.

Sorry, bad punctuation.
Should be:
I'm giving up. Eating chocolate for a month.

quiesco: I rest
Minimus has had a dies negotiosa (busy day) and he's fessus (tired) - he needs a little intermissio (pause) with a potio calida (hot drink)!
#LatinVerbs

Cheeky Scientist is a bad company that should be stopped.

Academic friends, please contact your department/school's career services and ask them to stop booking Isaiah Hankel or his company.

Read this important article by Catherine Offord, and pass it on

https://www.science.org/content/article/criticism-builds-against-ph-d-careers-firm-cheeky-scientist

The Swiss Government has officially launched their Mastodon server at https://social.admin.ch/

In their official press release the Government confirms it is a trial for one year. They state that "Mastodon has several characteristics that make it fundamentally attractive for government communications", such as being beyond the control of others, as well as it being privacy friendly.

Official press release: https://www.admin.ch/gov/de/start/dokumentation/medienmitteilungen.msg-id-97696.html

Speaker of the Swiss Government: @gov

#DarkSky International (formally #IDA) has announced the winners of it's 2023 Capture the Dark photography contest. For some beautiful dark sky photography, check out the finalists:
https://darksky.org/news/2023-capture-the-dark-winners/

My fav.
https://darksky.org/app/uploads/2023/08/1st-place_-1584_Creatures_of_the_Night_Petr_Horalek.jpg

"Don’t believe HR’s endless blather about “putting people first” or “breaking the silence about bullying”. It is just spin to enable your university to look good and get its Athena SWAN badge."
https://www.timeshighereducation.com/opinion/bullying-feature-uk-research-universities-not-bug

Iva Lučić shows our students how to label CaMKII with fluorescent dye for #singleMolecule TIRF #microscopy #biophysics

Thomas Korte shows the students how to use TIRF microscopy to visualise single molecules, and what a dirty coverslip looks like. #biophysics

🔬First method-focused work from our lab is just out in Cell Reports Methods 🎉
Interested in multicolor SMLM? Check the open-access final version of Karoline Friedl's PhD work, with Sandrine Lévêque-Fort's lab and the Abbelight gang
https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(23)00215-1

Have you read "The B Lane Swimmer?". If not, you're in for a treat: https://holly.witteman.ca/the-b-lane-swimmer/