Stephan Preibisch

Follow-up update for #BigStitcher is out! Now supports:
1) export of BigDataViewer/BigStitcher compatible HDF5/N5 datasets
2) export of #OpenSeadragon datasets for 2D images and 2D slices of 3D images

Stephan Preibisch

Exciting New Year's release of #BigStitcher! It now supports:

1) efficient blocked processing, meaning large, tiled acquisitions are fused blazingly fast (second/minutes instead of hours)!

2) Export of ZARR/N5/HDF5 thanks to the N5-API

Check it out, just update Fiji!

Stephan Preibisch

Exciting New Year's release of #BigStitcher! It now supports:

1) efficient blocked processing, meaning large, tiled acquisitions are fused blazingly fast (second/minutes instead of hours)!

2) Export of ZARR/N5/HDF5 thanks to the N5-API

Check it out, just update Fiji!

Albert Cardona

Now onto #FijiSc: Fiji is a recursive acronym meaning "Fiji is just ImageJ" fji.sc (and the paper nature.com/articles/nmeth.2019 ) –and #ImageJ is a #java open source software for image processing imagej.nih.gov/ij/index.html written by Wayne Rasband from the #NIH Research Branch.

An analogy: think of ImageJ as the kernel and Fiji as the rest of the operating system.

#FijiSc brings to #ImageJ:
(1) a package manager to install and update plugins, and that crucially enables reproducible science by exporting the whole set of plugins and libraries as an executable;
(2) a Script Editor imagej.net/scripting/script-ed supporting many languages (#python, #groovy #ruby #scala #clojure and more), all with access to a huge collection of #JVM libraries;
(3) huge amount of libraries such as #ImgLib2, #JFreeChart for plotting, for GUIs, etc.

There are many, many plugins. A tiny sample:

Machine learning-based image segmentation:
- #LabKit imagej.net/plugins/labkit/
- #WEKA Trainable Segmentation imagej.net/plugins/tws/index

3D/4D/ND Visualization:
- 3D/4D Viewer #3DViewer imagej.net/plugins/3d-viewer/i with ray-tracing, orthoslices, volume rendering, and more
- #BigDataViewer #BDV imagej.net/plugins/bdv/index for interactively navigate N-dimensional image volumes larger than RAM

Image registration and serial section alignment:
- #BigStitcher for registering 3D/4D tiled datasets, with multiview deconvolution and more imagej.net/plugins/bigstitcher
- #TrakEM2 for montaging in 2D and alinging in 3D collections of serial sections, typically from #vEM (volume electron microscopy) syn.mrc-lmb.cam.ac.uk/acardona
- #mpicbg libraries for extracting #SIFT and #MOPS features, then finding feature correspondences and estimating rigid and elastic transformation models nature.com/articles/nmeth.2072

Summarizing #FijiSc is impossible. See the online forum where questions find answers by the hand of the broader community of users and developers forum.image.sc/