Daniel Caron

Macrophages are essential immune cells that reside in all tissues. Some macrophages arise from monocytes in the blood, but during fetal development they also come from a unique pathway!

We still have a lot to learn, but here I'll describe how Zhao et al. (2023) used mouse models to uncover hints about which progenitor cells create tissue-macrophages.

doi.org/10.1016/j.celrep.2023.

#Macrophages #MouseModel #Immunology #Immunity #scRNAseq #FlowCytometry #Research #JournalArticle #JournalClub

1/9🧵

Daniel Caron

@biorxiv_bioinfo

Very cool #Preprint from the lab of Jo Jones at #UniversityOfCambridge I've never used #JuliaLang, but this appears to make a lot of the analyses that our lab has hard-coded into a very nifty interactive web-app!!

Really awesome way to make complex #FlowCytometry analysis accessible when built-in tools to #FlowJo don't quite deliver!

#Immunology

Daniel Caron

Really cool application of Infinity Flow to #MouseModel #Tcells, which allows readout/prediction of >200 surface epitopes on single cells via #FlowCytometry.

doi.org/10.1016/j.immuni.2023.

By @maxevrard
#Immunology #TissueImmunity

gittaca

@TRyanGregory #FlowCytometry is magic 🤩 So many natural science subjects in one device!

IMPACTT Microbiome

Sony Biotechnology is a sponsor of #HavingIMPACTT2023. Discover how Sony Biotechnology #flowcytometry solutions can help to advance your research at sonybiotechnology.com/us/

Thiago Carvalho

Tools (#preprint)

"Our platform is built around a commercial instrument and integrates the handling and transfer of samples to and from the instrument, autonomous control of the instrument's software, and the algorithmic generation of sorting gates, resulting in walkaway functionality."

#FACS #FlowCytometry

biorxiv.org/content/10.1101/20

An open-source FACS automation system for high-throughput cell biology

Recent advances in gene editing are enabling the engineering…

www.biorxiv.org
Tim Dobbs

Anybody know about anything cool happening in #FlowCytometry lately? I want to hear some neat stuff about #SingleCellAnalysis

DartLab

Time for an #Introduction

This is the mastodon account for DartLab, the Immune Monitoring and Flow Cytometry Shared Resource at the Dartmouth Cancer Center in Lebanon, New Hampshire.

We do work in #Immunology, #FlowCytometry, #Immunotherapy, #Cancer, #Autoimmunity, and #InfectiousDiseases (including #COVID). Looking forward to connecting with other #SharedResources and #CoreFacilities.

This account is run by @mielcarz .

MPI für Molekulare Genetik

⚡️ We are looking for a Research assistant (m/f/d) for our #FlowCytometry & #Cell Sorting group (headed by @MissesThiel). If you have XP in multiparameter flow cytometry & cell sorting by #FACS, check below & apply until February 18, 2023!

#berlinjob

molgen.mpg.de/4593631/job_full

Research assistant (m/f/d) | Flow Cytometry and Cell Sorting

We are seeking a highly motivated Flow Cytometry and…

www.molgen.mpg.de
DeNovix Inc.

Prior to downstream applications like single-cell sequencing, it is crucial to perform sample quality control analyses.

Using an automated cell counter—like the CellDrop FL—along with fluorescence viability dyes is a great way to ensure high quality nuclei isolations.

Download this resource to explore advances in quality control methods for single nuclei sample preparation.

denovix.com/ebook/automated-co

#FlowCytometry #CellBiology #Science #Genomics #Immunology #Fediscience

Arjan Boltjes

Assessment of #Tcell proliferation and other characteristics over a period of ten year. Wow! Fun read.

From the abstract by Soerens et al.: "These observations provide a model to better understand memory cell differentiation, exhaustion, cancer and ageing, and show that functionally competent T cells can retain the potential for extraordinary population expansion and longevity well beyond their organismal lifespan."

Found via @biomiha and @billjeffries

#RNAseq #FlowCytometry #Tcells

palomita

Are you interested in Immunology and do you want to come to Germany?
1 year Marie Curie Fellowship for a Predoctoral Researcher @James_Hutc@twitter.com
#macrophages #Tregs #FlowCytometry Please RT
See details
👇 👇 👇
euraxess.ec.europa.eu/jobs/593

First Stage Researcher (R1)

INsTRuCT is an Innovative Training Network (ITN) funded…

euraxess.ec.europa.eu
Physalia-courses

Many thanks to Dr. Alice Yue and all attendees for making the first edition of the Flow Cytometry analysis in R/Bioconductor a great success!

Best of luck with the analysis of your data!

#Rstats #Bioconductor #flowcytometry

Adrian Liston

If you can already use R, you can skip the tutorial and get the repository straight from GitHub. We have separate scripts optimised for #flowcytometry / #masscytometry or #scSeq. As well as the stats there are nice data visualisation tools included. 4/6 github.com/AdrianListon/Cross-

GitHub - AdrianListon/Cross-Entropy-test: Unified script for applying the t-SNE diff method on single cell RNA-seq data

Unified script for applying the t-SNE diff method on…

github.com