#fluorescencefriday

#FluorescenceFriday

Front yard
Not really fluorescence, but like fluorescence

#Flower #Fluorescence #Plant #Yard #Photography

#FluorescenceFriday

Some nice colors of Dragon fruit
This reminds me that I should plan some fluorescence microscopy experiments

#DragonFruit #Fruits #Photography #Fluorescence #Science #Experiment

New publication from Kwanghun Chung lab at MIT improves on their SWITCH approach and eFLASH stochastic electrotransport to improve antibody labeling by gradually shifting the microenvironment. Looks interesting, I wonder how well it can be adapted to other labeling protocols.

Uniform volumetric single-cell processing for organ-scale molecular phenotyping
Yun et al., Nature Biotechnology 2025
https://doi.org/10.1038/s41587-024-02533-4

#neuroscience #tissueclearing #lightsheet #microscopy #fluorescenceFriday

I missed #FluorescenceFriday but here's an image for one from the manuscripts on the nearly-finished pile. We've found a genetic marker for the deep CA1 pyramidal cells. Magenta is calbindin (IHC), cyan is the other cell type (AAV-TdTom). Less than 5% overlap. Coming to BioRxiv by Easter, hopefully! #Neuroscience

@MFM_BIC@synapse.cafe @MFM_BIC

ICYMI you can follow tags in here:

#FluorescenceFriday
#NeuroImaging
#CellBiology

On #FluorescenceFriday, we’re highlighting this beautiful SIM image from @dgaboriau These human fibroblasts are labelled to visualise mitochondria (Tom20) in yellow, alpha-tubulin in pink and actin in grey.
https://focalplane.biologists.com/2024/10/11/featured-image-with-david-gaboriau/

for #FluorescenceFriday, here's a video of two-photon imaging from mouse visual cortical neurons at 0.5x speed in response to many natural images #neuroscience (paper here: https://www.biorxiv.org/content/10.1101/2024.06.30.601394v1)

Happy #fluorescenceFriday, let’s see those gorgeous images!

#FluorescenceFriday

It’s #FluorescenceFriday & we’re highlighting Shivangi Verma’s (@shivangiv10) image of a #zebrafish larval brain. The larva expressing GCaMP6f was embedded in agarose & imaged using a Zeiss Lightsheet microscope. Find out more about Shivangi's research:
https://focalplane.biologists.com/2024/08/30/featured-image-with-shivangi-verma/

Happy #FluorescenceFriday

Some actin action always - amazes me (and gets me safely into the weekend)

Timelapse imaging on Zeiss880 of HEK cells expressing Lifeact-mEGFP
LUT is Cyan Hot (quite like that one)

#FluorescenceFriday
(is this a thing on #Mastodon - if not let's make it one!)

Nuclei labelled with EGFP-H2B.
MIP of a small z-stack at two frames per minute.

Loads of action, movement and cell divisions, in the developing zebra fish hindbrain

@focalplane_jcs prime #fluorescenceFriday material! #lightsheetmicroscopy creates such satisfying visuals :)

Hey, it's #FluorescenceFriday !!! Have a look at the fluorescence from the chlorophyll of these tiny little happy algae. Image courtesy of our oblique #lightsheet microscope! In their DYE-ART project the awesome @alessiacandeo and Barbara Menin are going to make these little guys super efficient at capturing CO2 from the atmosphere.

Happy #FluorescenceFriday and congratulations to Stéphanie Durand, Dept. of Chromosome Biology, Raph_Mercier lab, MPIPZ Cologne, Germany for her 2nd place in the Huygens Image Contest, with this high quality deconvolved STED MIP image of the synaptonemal complex at meiosis.
https://svi.nl/ImageContestWinners
#microscopy #bioimaging #scienceart #phdresearch #bioart

On #FluorescenceFriday get your vote* in for your favourite JCS cover from 2023! Voting is open until Monday 22 January.
https://focalplane.biologists.com/2024/01/12/vote-for-your-favourite-jcs-cover-from-2023/
* we also have some fantastic images that don't use fluorescence microscopy!!

Happy belated #FluorescenceFriday for those who celebrate! #RealTimeChem

Check out our fabulous #FluorescenceFriday featured image from Joe McKellar. In our post, we find out more about the image and Joe’s research.

focalplane.biologists.com/2023/12/08/featured-image-with-joe-mckellar/

Check out some #CoolScienceImages (with music by @kesha) from the #CSBCPSON23 #CancerResearch Meeting! #FluorescenceFriday

Music: Heartbreak Anthem by Galantis, David Guetta, & Little Mix

Here's a question for #ImagingScientist #Microscopy folk and #ImageAnalysis people (but also #grammar if that's your thing).

I'm using #DAPI, #SYTOX or something else to label the DNA in cells. I want to segment them based on this.

Am I doing:
A) Nuclei Detection
B) Nucleus Detection
C) Nuclear Detection
D) Other (please comment!)

Please boost and tag any #Imaging folks you know. I need to settle a dispute.

Wishing it was #FluorescenceFriday already.

happy #FluorescenceFriday from the omega cell!