#microscopy

I believe this might be a pollen grain from the lime trees that live by us...
#Science #Biology #Flora #Microscopy
40x obj / 12.5x cam TfT stain 460nm epi #Fluorescence

Ooooo What do we have here?
25x ThT 460nm epi #fluorescence .
#science #biology #microscopy

This is a rather fascinating artefact in the spider silk, what could it be?
#microscopy #science #biology #fluorescence #ThT #spider

Experimenting with #ThT stain on #spider webs to see if it successfully bonds to the beta chain #fibrin / #amyloid like components. I've applied the stain and naturally under 460nm webs fluoresce the expected green. I'm waiting for the sample to completely dry before differentiating with alcohol and then using a water based mount to finally disassociate the unbounded stain from the webs and other artefacts. All being so, we might be left with ThT stained #spidroins #science #biology #microscopy

If that isn't pollen, I'm a monkey's uncle... about 30 microns diameter.
100/1.32na objective 90na condenser.

#Microscopy #Science #Biology #Botany #Flora

I spent the day rectifying segmentation and tracking for bacteria in microfluidic devices. 1000 frames, 5 chambers, ~8 cells per chamber, so 40000 pictures of cells to look at, and check that they match frame to frame. I think I'm done with screens for the day. Fortunately automation helps, and drawing the lineages as graphs allows to spot errors more easily.
Now I have a clean dataset that I can analyse and also use as a training dataset for the next iteration!

#microscopy #AcademicChatter

I’m currently looking for a post-doc in the UK, particularly to work on projects that employ in-vitro reconstitution and quantitative #microscopy approaches to study #cellbiology processes (esspecially #membranetraffic) + would be grateful for sign-posting towards openings! #academicchatter

Congratulations to the top 3 images from the Node–FocalPlane image competition!

1. "Cell-estial bloom" by Özge Özgüç

2. "Dancing actinotroch" by Allan Carrillo-Baltodano

3. "Who’s active?" by Julia Peloggia de Castro

@the_node @focalplane_jcs

#biologists100 #biology #CellBiology #research #closeup #microscopy

Here's an interesting way to do photobleaching with cleared tissue: Add 0.1% H2O2 to the clearing medium and blast with LEDs for 24h at RT. Also includes very detailed instructions for building an LED photobleaching setup - the samples ate placed between two PCBs with LEDs in a ventilated box.

Open-source Photobleacher for Fluorescent Imaging of Large Pigment-Rich Tissues
Murakami et al., preprint at biorxiv 2025
https://www.biorxiv.org/content/10.1101/2025.02.24.639965v2.full.pdf

#tissueclearing #fluorescence #microscopy #neuroscience

I'd like to point to a possible error in some information regarding the use of ultraviolet (UV) light to illuminate samples of 'white macro clots' stained or unstained under the microscope...
#conspiracies #whiteclots #histology #microscopy #biology

Live-cell labeling sheds light on how our DNA is packed and behaves in cells

https://phys.org/news/2025-04-cell-dna-cells.html

#research #biology #science #dna #microscopy

(04 Apr) We have the first video of a plant cell wall being built

Plant cells without a cell wall are fragile, so it’s hard to image its construction.

https://s.faithcollapsing.com/tr6yl
Archive: ais: https://archive.md/wip/zLy3L ia: https://s.faithcollapsing.com/7264y

#biology #cell-wall #imaging #microscopy #plants #science

Welcome to the new edition of #nakeddiefriday everyone. The guest today is the famous ESP8266EX by Espressif, a SoC loved by many in the electronics world.

Unfortunately, the digital section is fully covered by two topmost metal layers carrying power. We do have a glorious analog/RF section though.

SiPron page: https://siliconpr0n.org/archive/doku.php?id=infosecdj:espressif:esp8266ex

#electronics #reverseengineering #microscopy

Wonderful discovery for #protists #independentresearch #microscopy #microbiology folks. Check out a new zine called "Amoeba Discovery":

https://stefanluketa.com/amoeba-discovery/

I never thought very much about protists/amoebas, and reading this volume was a fascinating introduction to what seems to be an understudied field.

Now I'm wondering how I might isolate and sequence some interesting samples!

@foaylward

Reflecting on #biologists100 last week, I greatly enjoyed meeting past colleagues and getting to know the work of @Co_Biologists better. The experience reinforced for me the importance of society own journals and their work.

From the programme, I especially enjoyed the talks of Jennifer Lippincott-Schwartz and Manu Prakash in the emerging technologies session, #microscopy is the future! (1/2).

Alright, let's get the #nakeddiefriday going.

Today's exhibit is AR9281 by Atheros, a very classic Wi-Fi chip found in many devices. Comes in very pink hues. A short thread with highlights follows.

SiPron page: https://siliconpr0n.org/archive/doku.php?id=infosecdj:atheros:ar9281-al1e

#electronics #reverseengineering #microscopy

It's such a pleasure to work with great equipment. Askania SMT4 microscope, Leica Flexacam C5, Robin's tools pixelpump.

@leicamicrosystems @robin1337
#Askania #smd #smt #soldering #rework #tools #microscopy #pixelpump #leica
#MadeinEU #madeingermany

New #microscopy technique follows individual molecules as they pass through a nuclear pore complex in a live cell. Resolution of nanometers and milliseconds. https://www.nature.com/articles/d41586-025-00804-x