#Parasitology paperwatch: recent work from
De Graffenried lab (FA: RS Muniz, PC Campbell) adapting a live-cell #imaging technique from bacteria to #trypanosomes, namely confinement of single cells to agarose microcells.
They show how the ability to track single cells over the entire cell cycle can lead to new insights. Show specifically that the 2 daughter cells produced after cell division go on to divide at different time (the one with the old flagellum is faster)
Also address recent controversy regarding a putative "backup cytokinesis" pathway triggered in TOEFAZ1/CIF1 RNAi, & show that the proposed pathway is not operating 🤩. Good description of how setup & imaging conditions were optimised & excellent suggestions for future applications of the approach😃👏👍
