In the process of simplifying the Circle-Seq method, we realized that our method offers much more: it can also find out if the pieces of donor DNAs (used for inserting a gene at the CRISPR cut site) are inserted correctly and if they are inserted elsewhere (random insertions).
Random insertions are identified by strategically placing the guide RNAs (gRNAs) used in the CRISPR-KRISPR assay: one gRNA in one of the homology arms and the second binding to an overlapping region between the insert and the other arm. See article for more details.
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The CRISPR-KRISPR method can also be useful for characterizing knock-in loci in other model organisms, including cell lines!
