Manuel Vicente @ManuelMVicente@qoto.org

(11/13) Finally, these mice showed spontaneous features of inflammation and were more susceptible to experimental inflammatory disease

(10/13) Defects included impaired ß-selection, thymic Treg generation and altered TCRvß repertoires in CD8 SP thymocytes

(9/13) These mice had severe developmental defects, when compared to their littermate controls

(8/13) Amazed by these glycome dynamics, we then generated Mgat1 conditional knockout mice, using a Rag1Cre line, having a blockade in the N-glycosylation pathway early in development

(7/13) In addition, we analyzed available scRNA-seq data from human and murine thymocytes and observed that key glycogenes’ expression levels displayed the same dynamic developmental features as the lectin binding levels

(6/13) In our work we show that the glycocalyx composition is dynamic across developing thymocytes, both in human and mice, using plant lectins