Hi fellow humans, I am Laura (zie/zir if possible, otherwise she/her), a senior staff scientist at #LSFOctopus advanced #LightMicroscopy and #CLEM facility at #HarwellCampus in the #UK, right next to #DiamondLightSource.
My science interest center around #EGFRSignalling and #RTKSignalling and deregulation in #CancerStudies in general. My tools of the trade are #singleparticletracking #singlemoleculelocalisation #CLEM #FIBSEM #VolumeEM and #CryoMicroscopy
HMU for science chat
@lauraczd Hi Laura! Can I ask what type of CLEM you’re doing? We’re thinking about some CLEM experiments using Tokuyasu sections at the moment so interested to hear from anyone else in Oxford using the technique!
@rbeagrie Hi Rob, we have a Zeiss Crossbeam with a Quorum cryostage for cryo FIB-SEM volume imaging.
I have never tried your technique, but I know it's quite challenging.
What are you trying to image?
@lauraczd What we’re planning to do would probably be more fairly described as semi-correlative. We’ve got a population of cells where around 50% have a distinctive chromatin rearrangement in the nucleus that can only be identified by EM. We want to see if that phenotype is associated with a different distribution of various nuclear proteins. So the EM part is mostly just to identify cells of interest.
@rbeagrie oh wow, that sounds very interesting and potentially very challenging.
What does the chromatin look like in the state you're interested in?
Maybe there is a fluorescent probe that can report on that either through intensity or lifetime?