Building on work of Harer \cite{Ha86}, we construct a spine for the decorated Teichmüller space of a non-orientable surface with at least one puncture and negative Euler characteristic. We compute its dimension, and show that the deformation retraction onto this spine is equivariant with respect to the pure mapping class group of the non-orientable surface. As a consequence, we obtain a model for the classifying space for proper actions of the pure mapping class group of a punctured non-orientable surface, which is of minimal dimension in the case there is a single puncture.