#Telomeres #Telomerase #tumorigenesis #Preprint from Charles Kinzig, Titia de Lange et al
"Here, we aimed to determine whether telomerase can add telomeric DNA to DSBs in human cells and how this process is regulated. The data indicate that telomerase can create a functional neotelomere at a Cas9-induced DSB that bears the TS sequence at one 3′ end. The frequency of neotelomere formation is increased upon overexpression of telomerase, suggesting that the low level of telomerase in most human cells minimizes these deleterious events. In addition, neotelomere formation is inhibited by ATR signaling at resected DSBs. We discuss these findings in the context of genome instability during tumorigenesis, where neotelomere formation by telomerase might end BFB cycles as originally proposed by McClintock."
