"A major challenge is that relatively few enhancers are functionally characterized in vivo, making it difficult to draw generalized conclusions about enhancer-promoter looping during developmental gene activation. Here, we investigate the 3D conformation of enhancers during mammalian development by generating high-resolution tissue-resolved contact maps for nearly a thousand mammalian enhancers with known in vivo activities in ten murine embryonic tissues. We performed enhancer knockouts in mice, which validated newly identified enhancer-promoter chromatin interactions. "

#MolecularBiology #GeneRegulation #Enhancer #preprint

https://www.biorxiv.org/content/10.1101/2022.11.18.516017v1?med=mas