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"The (re)appreciation that true insight into neural function depends reciprocally on an in-depth understanding of the underlying structure(s) has driven a surge in efforts to develop novel imaging techniques. In fact, keeping track of these approaches, which continue to emerge at a staggering pace, has become a challenge by itself."
#neuroscience @PLOSBiology
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3002221
@cyrilpedia @PLOSBiology
Amusing that the piece includes optical microscopy methods unrelated to neural function, such as STED, PALM, expansion microscopy and CLARITY, but ignores electron microscopy methods key to the current boom in #connectomics. In particular, Denk and Horstmann 2004 for SBEM https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0020329 , Knott et al. 2008 for first application of FIBSEM to neuronal tissue https://www.jneurosci.org/content/28/12/2959 , Schalek et al. 2011 for the ATUM serial section tape-collecting system https://academic.oup.com/mam/article-abstract/17/S2/966/6947317 , Xu et al. 2017 for e-FIBSEM and Graham et al. 2019 for GridTape TEM https://www.biorxiv.org/content/10.1101/657346.abstract
#neuroscience #microscopy #VolumeEM #vEM