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'However, the established use of viral vectors for genetic engineering of CAR immune cells is still subject to particular safety concerns and requires further improvements. 1 Consequently, non-viral methods for immune cell engineering are gaining increasing interest. In this context, transposon-based technologies represent a promising alternative engineering technology that enables safe genomic insertions of large cargo, such as multi-cistronic CAR-expression cassettes, using fast and cost-effective manufacturing processes '

cell.com/molecular-therapy-fam

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