The Ishango Bone, discovered in 1950 near the Semliki River in present-day Democratic Republic of Congo and dated to over 24,000 years ago, features a series of 16 grouped numerical notches arranged across three columns. The interpretation of these markings has long been debated, ranging from numerical intention to decorative symbolism. This study presents a newly identified structural pattern, uncovering four irregularities in the numerical groupings that, when resolved, reveal a consistent internal logic across all three columns. The analysis identifies repeating sums, dualistic pairing structures, and cross-column symmetry, pointing toward a deliberate and complex mathematical arrangement. These findings suggest the notches may have functioned as a form of reference system, potentially using stone markers for visual or narrative purposes. Hypotheses include links to cosmic cycles, mathematical instruction, or mythological integration. While interpretations remain speculative, this study argues for an interdisciplinary reevaluation of the Ishango Bone's significance in terms of mathematical, cultural, and cosmological understanding. It offers new insights into the symbolic thinking and cognitive capacities of prehistoric societies and challenges conventional assumptions about the origins of numeracy.