Impact of Oxygen on DNA Damage Distribution in 3D Genome and Its Correlation to Oxygen Enhancement Ratio under High LET IrradiationThe variation of the oxygen enhancement ratio (OER) across different values of Linear Energy Transfer (LET) currently lacks a comprehensive mechanistic interpretation and a mechanistic model. Our earlier research revealed a significant correlation between the distribution of double-strand breaks (DSBs) within the 3D genome and radiation-induced cell death, which offers valuable insights into the oxygen effect. In this study, we formulate a model where the reaction of oxygen is represented as the probability of inducing DNA strand breaks. Then it is integrated into a track-structure Monte Carlo simulation to investigate the impact of oxygen on the spatial distribution of DSBs within the 3D genome. Results show that the incidence ratios of clustered DSBs in a single topologically associating domain (TAD) (case 2) and DSBs in frequently-interacting TADs (case 3) under aerobic and hypoxic conditions closely align with the trend of the OER of cell survival across various LET values. By utilizing the parameters derived from our previous study, we calculate the OER values related to cell survival. Our OER curves exhibit good correspondence with experimental data. This study provides a potentially mechanistic explanation for the changes in OER across different LET levels. High-LET irradiation leads to dense ionization events, resulting in an overabundance of lesions that readily induce case 2 and case 3. The probabilities of cell death associated with case 2 and case 3 are substantially higher than other damage patterns. This may contribute to the main mechanism governing the variation of OER for high LET. Our study further underscores the importance of the DSB distribution within the 3D genome in the context of radiation-induced cell death. This study also provides valuable reference points for establishing a mechanistic model of OER.
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