Tools #Organoid #CRISPR #preprint

"However, to date, the editing efficiency of organoids with the use of non-viral electroporation methods has been only up to 30%, with implications for the subsequent need for selection including including turnaround time and exhaustion or adaptation of the organoid population. Here, we describe an efficient method of intestinal organoid editing using a Ribonucleoprotein CRISPR-based approach."

https://www.biorxiv.org/content/10.1101/2023.03.31.535108v1