'The collection of practical guidelines and recommendations described here is primarily intended to facilitate the assessment of cellular senescence markers in living animals (in vivo) and in situ. Finally, this study focuses on cellular senescence research and does not aim to provide information on overcoming the general technological limitations of methodologies, such as autofluorescence for immunohistofluorescence (IHF) or mixed cell populations in tissue homogenates used for RT-qPCR or western blot (WB).'
https://www.sciencedirect.com/science/article/pii/S0092867424006408