Does anyone know of software for taking a set of images of a sample taken at different focal planes through an optical microscope, with Z axis position encoded in the filename, and reconstructing a 3D model based on which image a given feature is focused best in?
@azonenberg So, focus stacking, but using image metadata instead of relying on visual detection of sharpness? No idea how to do that, I think you would need a 3D model of the subject to generate the mask.
@miermont No the opposite. Use sharpness to stack then extract a 3d model from the focal plane masks.
@azonenberg That seems like an active research topic. I hope you'll find something usable.
@miermont Mathematica can do it https://www.wolfram.com/language/12/image-computation-for-microscopy/3d-reconstruction-via-focus-stacking.html?product=mathematica
Whether there's anything f/oss for Linux that can do the same is another question.
@miermont Yeah its not at all an urgent need but I'll keep an eye open.
Basically I'm already used to navigating samples in 3D by focusing to figure out which features are above/below others intuitively. Seems like something you could do computationally to 3D pinpoint specific features, I just don't know how to do it.