@preibischs @naturemethods Congratulations! But why the weird undersell in the size department?

Owing to current limitations in Java arrays, the theoretical upper limit is 2^31 = 2,147,483,648 blocks, with each block maximally containing 2^31 = 2,147,483,648 pixels (for example 2048 × 2048 × 512 pixels). Given sufficient storage and compute resources, the limit for RS-FISH is thus 4,072 peta-pixels (4,072 petabytes at 8 bit, or 8,144 petabytes at 16 bit) taking into account the overhead, whereas every individual block locally processes only 2 gigapixels (2^31 = 2,147,483,648 pixels).

1. 2G^2 is 4exa, not 4peta
2. ImgLib2’s CellImg manages 2^63 blocks, not 2^31, we had to change this for @davi ‘s FAFB which had >2^31 blocks.