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I have a question about image processing. I took images with the same magnification and zoom. I rotated and cropped them in FIJI and added scale bars. The scale bar on one of the images is longer than the others. What is going on. I am literally about to lose it. I appreciate any advice.#microscopy #FIJI#science
@steveroyle Thanks will try that! Crop sizes are the same and scale bars are being added in Fiji
@Drosmel assuming the crop size is the same for the images and that you are adding scale bars in Fiji (using one of the options and not manually), I'd suggest checking the scaling of the crops using the “Set Scale…” command. It is possible during some procedures that this scaling is lost/altered.