Jay @Blue_Jay@qoto.org

Felt like a real-life #Scientist today; I made a “cloud”

I have been working on protein purification. When clearing my lysate, the cleared lysate was under a layer of what I think is DNA. Does anyone have any suggestions on separating the DNA interfacing layer away from the cleared lysate?

I pipetted off the white layer off and saved it, but between the two tubes the layer was about 4 mL. Which seems like a lot of volume, and I don't want to lose the protein in that 4 mL.

My #introduction is that I'm a 3rd year graduate student in #biochemistry and #molecularbiology .
I study the RAG1/2 recombinase and its binding motif, which facilitates V(D)J recombination. I am actively trying to improve my knowledge and use of #programing #datascience and #machinelearning . Though I am fairly new to programing in general.

I have Functional Movement Disorder, which causes me to have episodes of shaking and various other symptoms.

I enjoy growing #plants and spending time in my #greenhouse and in the outdoors. I grow succulents, cactus, tropicals, and orchids.