Jay @Blue_Jay@qoto.org
I love plants and science, can I say more?
Disabled - Gay - Graduate Student
#Biochemistry #MolecularBiology #Plants
I study the RAG1/2 recombinase
Joined Nov 2022
Would teleportation technology mean you could make anything?
@aebrockwell Those are some great points! I like the idea of the scanned data being a backup life.
As long as the scanned data can be stored and is stable, It could be an alternative to cryopreservation. You just get scanned and reassembled 50 years later.
Would teleportation technology mean you could make anything?
@lucifargundam That's a bummer 😂
Would teleportation technology mean you could make anything?
A possible way to do teleportation is to scan, disassemble, then assembling the atoms of a object in the same order as the scanned object.
Would that mean we could just duplicate the thing by assembling it over and over, without rescanning? I am thinking you could just print endless food or just print anything that’s been scanned before. 🤷
Jay
boosted
On a positive note, today I submitted my draft post-doctoral proposal, which builds on my Ph.D. dissertation. In addition, my dissertation is now about 90% complete and I have the body written, now just need to get the conclusion and defense going. Things are moving! #WxMastodon #SciComm #severeweather #phdlife #academicmastodon
precipitated DNA in protein purification lysate
I have been working on protein purification. When clearing my lysate, the cleared lysate was under a layer of what I think is DNA. Does anyone have any suggestions on separating the DNA interfacing layer away from the cleared lysate?
I pipetted off the white layer off and saved it, but between the two tubes the layer was about 4 mL. Which seems like a lot of volume, and I don't want to lose the protein in that 4 mL.
First co-suthorship, so exciting!
This week, my lab recently published, and its my first co-authorship on a paper! 🎉
Our lab developed a high-throughput plasmid-based recombination assay, SARP-seq, to investigate RAG1/2's recombination of thousands of different DNA motifs.
The RAG1/2 recombinase facilitates the diverse antigen binding repertoire for the adaptive immune system, but can also aberrantly cause genomic instability.
Walker Hoolehan, Justin C Harris, Jennifer N Byrum, Destiny A Simpson, Karla K Rodgers, An updated definition of V(D)J recombination signal sequences revealed by high-throughput recombination assays, Nucleic Acids Research, 2022;, gkac1038, https://doi.org/10.1093/nar/gkac1038
@jadebees I have seen lots of people during winter harvest the pupae from the tubes, wash them off, and overwinter them. Does this encourage an increased survivability?
Jay
boosted
Have you used `subplot_mosaic()` in #Python's #Matplotlib yet?
Or are you a dinosaur like me and still use older functions? After all, `subplot_mosaic()` was only introduced in 2020 in version 3.3
Recently, I decided to finally explore `subplot_mosaic()` and I know I'll never go back to whatever I did before to plot these types of figures!
<code in next post>
/1
#coding #programming #Visualisation #LearnPython #LearnToCode
@trinsec @jamesadams I was really hoping for 65537 characters 😭
@scottbarolo oh my apologies, I wasn't familiar with the term.
@scottbarolo
Hey I am on my phone and couldn't find a place to add a comment. But OUHSC has the the GPiBS program. An umbrella programs covering like 7 departments. They offer six 6-week rotations
https://graduate.ouhsc.edu/Graduate-Programs/PhD-Programs/Graduate-Program-in-Biomedical-Sciences
@borak_faith it isn't the fastest way to get fruit but what fun it is to watch. Your orange babies look so nice!
Jay
boosted
Trying to figure this site out and find a chemistry teacher network here. Hello world? #edutooter
Jay
boosted
One of the most underrated #Python modules?
**`turtle`**
We know it's used for teaching & not in the "real-world"
But it's usually used only for basic drawings & simple animations for kids–a very limited scope
_We can teach more advanced topics, too…_
Here are some examples…
---
# 1. Data structures: lists, tuples, dictionaries, and sets
Here's an animation that uses all four of these structures:
_[Practise Using Lists, Tuples, Dictionaries, and Sets in Python With the Chaotic Balls Animation](https://thepythoncodingbook.com/2021/10/31/using-lists-tuples-dictionaries-and-sets-in-python/)_
---
# 2. Named Tuples
And a bit further down the line, here's an example of a `turtle` animation of a sunrise that introduces Named Tuples:
_[Sunrise: A Python Turtle Animation using Named Tuples](https://thepythoncodingbook.com/2022/06/12/python-turtle-animation-using-named-tuples-sunrise/)_
---
# 3. Demonstrating real-world processes
Maybe we want to use it model real-world processes in a quick-and-simple way.
Here is a demonstration of "simulating" bouncing balls
There's the single ball version:
_[The Real World in Python: Coding A Bouncing Ball](https://thepythoncodingbook.com/2021/08/19/simulating-a-bouncing-ball-in-python/)_
and the many balls one which deals with **object-oriented programming**:
_[Bouncing Balls Using Object-Oriented Programming in Python](https://thepythoncodingbook.com/2021/09/09/using-object-oriented-programming-in-python-bouncing-balls/)
And, one of my favourites, a simulations of planets orbiting one or more stars. The `turtle` version is in 2D:
_[Simulating Orbiting Planets in a Solar System Using Python](https://thepythoncodingbook.com/2021/09/29/simulating-orbiting-planets-in-a-solar-system-using-python-orbiting-planets-series-1/)_
_There's also a 3D version which uses Matplotlib instead of turtle, but that's off-topic here so I'll post another time_
---
Here are some more ideas from projects I've written up recently
https://thepythoncodingbook.com/category/turtle/
I have loads and loads more which I'll try to write up and publish in the coming months
#animations #python #turtle #coding #programming #LearnToCode #LearnPython
Jay
boosted
Whoa! I know bats are important, but this is insane. An exclusion experiment by Beilke et al shows that forests without bats are *three to five times* as insect-eaten as forests with bats.
@alexkwan a few weeks ago I heard a researcher on NPR talk about their studies of the mechanism of psilocybin. If I remember correctly, they said it seems to make the brain more plastic or something, and were using it as treatment for depression and ptsd.
But it made me wonder. Their are neuropsychological disorders which are described as when the brain utilizes an incorrect pathway to do a task, Like my functional movement disorder. Many clinics I went to a core in their treatment was reteaching your body/brain normal movements and the correct pathways to use. (lots of Occupational and physical therapy)
Since listening to that npr episode, I have been curious if treatment with psychoactive substances could potentially aid in the reshaping of those incorrectly used brain pathways.
@HemeOnc @War_Kittens how? It gave me a headache yesterday. I had barely any time to find three words in a post. If I was interested I ctrl + F the words I remembered from the post. 😂
@mainzebra maybe.
My #introduction is that I'm a 3rd year graduate student in #biochemistry and #molecularbiology .
I study the RAG1/2 recombinase and its binding motif, which facilitates V(D)J recombination. I am actively trying to improve my knowledge and use of #programing #datascience and #machinelearning . Though I am fairly new to programing in general.
I have Functional Movement Disorder, which causes me to have episodes of shaking and various other symptoms.
I enjoy growing #plants and spending time in my #greenhouse and in the outdoors. I grow succulents, cactus, tropicals, and orchids.
I love plants and science, can I say more?
Disabled - Gay - Graduate Student
#Biochemistry #MolecularBiology #Plants
I study the RAG1/2 recombinase
Joined Nov 2022
