Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro
An #OpenAccess T&R article from Oleksandr Kirsanov, Christopher Geyer and colleagues
This article is available under our Read & Publish Open Access initiative.
Researchers can find out about the benefits, read what researchers are saying and view a list of participating institutions at https://www.biologists.com/library-hub/read-publish/researchers/
Hi #ScienceMastodon. Have you already seen the latest post @preLights? 📜
It has been written by #AarrenMannion #KarolinskaInstitutet who covers the #preprint from the lab of Anne-Clémence Vion working at the #InstitutThorax. In this work, the team of researchers show the importance of the guanine nucleotide exchange factor, ARHGEF18, in regulating endothelial cell alignment in the direction of flow. 🌊
Hi #MedMastodon! 👋
#Introduction: I'm an #Endocrinologist at #Stanford, specializing in #Osteoporosis and #MetabolicBoneDisease. My lab studies #StemCells in bone 🦴 and the bone marrow microenvironment.
Some of my favorite topics: #WomenInMedicine #WomenInSTEM #PhysicianScientists #AAPI
I guess I should introduce myself #onhere: I am a postdoc in the Schier Lab at the Biozentrum (Switzerland). I am using #zebrafish to understand the #development of the nervous system. I love developmental biology of classic and non-canonical organisms, microscopy, loud music and comic books. Very much looking forward to meeting other scientists and cool people!
PS: I also help co-managing @ZebrafishRock , so make sure to give them a follow for the latest zebrafish community news
Evidence on #EndMT in human #MultipleSclerosis
Inflammation-induced endothelial to mesenchymal transition promotes brain endothelial cell dysfunction & occurs during multiple sclerosis pathophysiology
Dr. Helga de Vries lab Cell Death Dis 2019
https://www.nature.com/articles/s41419-018-1294-2
#EndothelialCell ARF6 GTPase links IL-1β inflammation with TGFβ/BMP-SMAD1/5 #EndMT
EC Arf6 KO in🐭#EAE->
⏫#BBB #BloodSpinalCordBarrier
⏬T cell infiltration & demyelination
w/o altering normal immunity
Dr. Shannon Odelberg, Weiquan Zhu labs Neuron 2022
https://www.sciencedirect.com/science/article/pii/S0896627322006602
Issue 21 is complete!
On the cover is a 3D surface rendering of a lung lobe from a mouse #embryo, showing the relationship between the arterial network (red) and the lung epithelium (grey) from Chandrasekaran et al.
Also in Issue 21:
- 8 #Research Highlights
- In #preprints article on reconstituting the ovary
- Ruth Bellairs obituary
- 2 author interviews
- Poster & article on resolving #morphogenesis into quantifiable cell behaviours
- Nuclear movement Review
Meet the #preLighters: Chee Kiang (Ethan) Ewe. 🌟
https://prelights.biologists.com/news/meet-the-prelighters-a-virtual-chat-with-chee-kiang-ethan-ewe/
Ethan is a #postdoc in the lab of @Odedrechavi at Tel Aviv University, Israel, where he studies the molecular basis of epigenetic #inheritance.
We spoke about his very international #career, the #mentors along the way, his future #goals, and the importance of #preprints both to him and the biological #community.
Ooh another really interesting paper on #alzheimers and #lactadherin
Medin co-aggregates with vascular amyloid-β in Alzheimer’s disease
Phospholipase Cε #Aortopathy
Plce1 KO🐭 are susceptible to ANGII-induced ascending #AorticAneurysm+Dissection
Role of adventitial myofibroblast cytokines?😎
Dr. Alan Smrcka, Santhi Ganesh, Markus Bitzer labs AJP Heart Circ 2022
https://journals.physiology.org/doi/abs/10.1152/ajpheart.00262.2022
A new #SpatialTranscriptomics technique was published on Cell! The authors used polony gels to capture the RNA in situ, and used pixel-seq to map #singlecell transcriptomes back to the tissue. Amazing experimental design! https://www.cell.com/cell/fulltext/S0092-8674(22)01367-8
BulkSignalR, a R package to infer ligand-receptor networks from bulk data including Visium #rstat #RNAseq #SpatialTranscriptomics
https://www.biorxiv.org/content/10.1101/2022.11.17.516911v1
scFates: a python package for pseudotime analysis from single cell data #scRNAseq #scATACseq #bioinformatics #python
https://academic.oup.com/bioinformatics/advance-article/doi/10.1093/bioinformatics/btac746/6832042
Kidney epithelial #Tubuloid
CD24+ epithelial progenitor-derived
4-phase protocol, Matrigel +Wnt3a/RSPO1, grow > 6 mo
CRISPR modeling #ADPKD, Cyst size responsive to #Tolvaptan
Super-impressive single-cell or single-nuclei RNAseq analyses to dissect the spatiotemporal molecular/cellular dynamics of this cool renal tubuloid, laying a critical foundation to enhance its clinical utilities👍
Wonderful #Lumenogenesis of CD24+ epithelial progenitor-derived nephrospheres, day 7 in Matrigel🤠
Dr. Rafael Kramann lab @NatureGenet 2022 @jackxuyx @KuppeChristoph @rkramann
https://www.nature.com/articles/s41588-022-01202-z
Welcome @karinayaniv ! I’m also just testing the waters here. I like it :) Slowly but surely finding all ours friends and colleagues! #science
@Natashaobrown
Really happy that you like it😆
Slides of my talk "An ABC of fluorescent proteins: Advances, Brightness and Considerations" presented at Symposium of the Microscopy Imaging Center (MIC) in Bern 2022 are available on Zenodo: https://doi.org/10.5281/zenodo.7341801
Editing a gateway for #CellTherapy across #BloodBrainBarrier
An excellent review🤠 Learn a lot!
Lesson from Leukocyte-#BBB interaction->
Engineering strategies->
⏫Cell delivery to injured🧠#Stroke
Dr. Rust Ruslan lab Brain 2022
https://academic.oup.com/brain/advance-article/doi/10.1093/brain/awac393/6832391
#EndothelialCell MEK1 #VascularMalformation
P1 topical 4OHT-induced mosaic EC MEK1 K57N transgene (what is the efficiency🧐)->
50% death by P30 +multiorgan VM (Arteriovenous malformation or other lesions) & Hemorrhage
Lesions composed of both mutant & WT EC
Dr. Patrick Smits lab Angiogenesis 2022
https://link.springer.com/article/10.1007/s10456-022-09853-6
Cardiovascular biologist, Dr. J. Geoffrey Pickering lab, Robarts Research Institute, Western University, London, Ontario, Canada
https://scholar.google.ca/citations?hl=en&user=IbrDx00AAAAJ&view_op