Isolation of mitochondria is typically a laborious process requiring single-sample processing with Dounce homogenization. The [Mitochondria Isolation Kit](https://www.thermofisher.com/order/catalog/product/89874#/89874) uses a non-mechanical, reagent-based method that allows multiple (six) cultured cell samples to be processed concurrently in about 40 minutes.
Isolated mitochondria can produce ATP from the oxidation of pyruvate to CO2 and water, provided they are adequately supplied with ADP, inorganic phosphate, and O2.
If the cells of an organ such as the liver are broken apart and their contents mixed together in a test tube, chemical chaos results, and the cells’ enzymes and other proteins are quickly degraded by their own proteolytic enzymes.
sources/citations: i once wrote an article rating which president is most likely to kill me in a fist-fight that is based on empirical evidence and truth of the universe so i know what im talking about
A #mastodon exclusive! You are invited to play on a *REAL* PDP-8/e running the TSS/8 monitor (TimeSharing System 8) pictured here. There is a user contributed tutorial here:
To connect, ssh email@example.com
If you are unable to connect that means all 6 (yes, just 6) lines are in use. Remember, this is real hardware and there is NO EMULATION happening. It is the real deal! Enjoy!
Melting roads are the environmental/infrastructure Y2K bug. Melting point.... it’ll be fine...
Why do science advocates listen to Bill Maher? Answer: They don't.
@binsrc it boils down to fast approximation vs slow precise answer. When you are constrained on time, you just can't be slow. In teaching the constraint could be the amount of attention the student is willing to pay before getting any tangible results/gains.
The point of what's more correct becomes moot, especially when you realize that most practical applications of mathematical theories are fast approximations.
autonomous, self-propagating, chemical system, low intelligence quotient, possible synaptic dysfunction, flawed sense of humor, poor genetic material, reproduction not recommended. 1 out of 10 stars.
QOTO: Question Others to Teach Ourselves
An inclusive, Academic Freedom, instance
All cultures welcome.
Hate speech and harassment strictly forbidden.