Hao Yin @HaoYin@qoto.org

Fantastic story
AT1-#EndothelialCell crosstalk in Lung #Alveologenesis🤠

Hopx+ AT1 Vegfa KO->
Loss of Car4+ EC ramifying over Alveoli
w/o reduced PLVAP+ capillary EC->
Alveolar Smoothening (⏬Outpocketing?)🧐

Dr. Jichao Chen lab Dev Cell 2020
https://www.cell.com/developmental-cell/fulltext/S1534-5807(20)30010-1

Arterial dissections: Common features and new perspectives

A comprehensive review covering diverse arterial beds

Biomechanics
#EndothelialCell+#SmoothMuscleCell plasticity
Genetics
TGFβ, ECM

Dr. Robert Graham lab Frontiers Cardiovascular Medicine 2022
https://www.frontiersin.org/articles/10.3389/fcvm.2022.1055862/full

Mouse Lung Spliceomic e15.5-P8

Enriched pathways Junctions; Hippo

Novel VEGFA splice variant
VEGFA i5😎

AT1 as a major lung cell for VEGFA expression & alternative splicing

VEGFA-188 (not -164) promote Car4+#Aerocyte specification

Dr. Francisca Vasconcelos & Cláudio Franco labs, Life Science Alliance 2022
https://www.life-science-alliance.org/content/5/12/e202201554

Lung #EndothelialCell Heterogeneity & Proliferation post H1N1 #Influenza injury

EC subpopulation 14 dpi trajectory (Slingshot)
Ligand-receptor analysis (FANTOM5)->
AT1-to-Car4-hi EC signaling in #AlveolarRegeneration

Dr. Edward E Morrisey lab @eLife 2020
https://elifesciences.org/articles/53072

Stabilizing FOXF1 to treat #AlveolarCapillaryDysplasia

Chemical screening->TanFe
⏬Ubiquitination of FOXF1 via⏬binding to #E3Ligase HECTD1/SHPRH/NEURL1B etc->

Restoring Lung #Angiogenesis of Foxf1+/-🐭

Dr. Vladimir Kalinichenko lab Am J Respir Crit Care Med 2022
https://www.atsjournals.org/doi/10.1164/rccm.202207-1332OC

How FOXF1 impacts #EndothelialCell #Angiogenesis?

As a transcription factor for S1PR1 & VE-Cad😎

An excellent story from Dr. Vladimir Kalinichenko lab @scisignal 2016👇

FOXF1 maintains endothelial barrier function and prevents edema after lung injury

https://www.science.org/doi/10.1126/scisignal.aad1899

Alveolar Capillary Dysplasia with Misalignment of Pulmonary Veins, due to FOXF1 heterozygous mutation, has very complex Lung & ex-Lung vascular phenotypes

A few reads🤠

https://diagnosticpathology.biomedcentral.com/articles/10.1186/s13000-020-00972-6

https://www.nature.com/articles/jp201494

https://www.nature.com/articles/ejhg2012171

https://www.jpeds.com/article/S0022-3476(04)00647-X/fulltext

Aberrant Mechanical tension-activated TGFβ1-SMAD2/3 signaling, synergized with Cdc42 deficiency, in AT2 alveolar cells causes peripheral-to-center progression #PulmonaryFibrosis in🐭post-lung lobe resection

Dr. Jingyu Chen & Nan Tang labs @cellpress 2019
https://www.cell.com/cell/fulltext/S0092-8674(19)31284-X

Interesting, ablation of TGFβR2 in Cdc42 KO AT2 cells blunts fibrotic expansion w/o rescuing #AlveolarRegeneration

In contrast, Normalizing the ectopic mechanical tension post-lung lobe resection, using a unique silicon prosthesis, rescues both alveolar regeneration & fibrosis

How Cdc42 controls AT2 cell mechanosensitivity & plasticity during #AlveolarRegeneration?

A Cdc42-F-actin-JNK/p38-YAP cascade

A very cool paper🤠👇

Dr. Nan Tang lab @CellReports 2016
https://www.cell.com/cell-reports/fulltext/S2211-1247(16)30919-6

Single-cell Transcriptomic Atlas of human Lung

75k cells
58 molecular types: 15 epithelial, 9 endothelial, 9 stromal, 25 immune
7 tissue blocks
1♀️+2♂️

17/58👤molecular types absent in🐭 Lung

Dr. Stephen Quake & Mark Krasnow labs @Nature 2020
https://www.nature.com/articles/s41586-020-2922-4

AT1 cell Plasticity in #Alveologenesis & Lung Homestasis+Regeneration

AT1 contributes to 30% & 10% AT2 in neonatal & adult 🐭Hyperoxia Lung injury👹

TAZ (likely > #YAP) maintains adult AT1 lineage identity Fig 6E

Dr. David Frank & Edward Morrisey Labs @CellStemCell 2021
https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00184-3

Vinculin-α-Catenin complex⏫#EndothelialCell Barrier during vascular morphogenesis

Vinculin a/b deficient🐟 48hpf->
Enhanced Permeability of 10 kD (not 70 kD) dextran of DLAV & ISV, but not dorsal aorta

Dr. Stephan Huveneers lab Vascular Biology 2022
https://vb.bioscientifica.com/view/journals/vb/aop/vb-22-0012/vb-22-0012.xml

Causal role of #Endotheliopathy in #Emphysema #COPD 🤠

i.v. Delivery of E4ORF1-transduced Lung #EndothelialCell
Or EC LRG1 (TGFβ/Alk1/Eng modulator) KO
alleviates elastase-induced🐭COPD

Dr. Alexandra Racanelli, Shahin Rafii & Augustine Choi labs J Exp Med 2021
https://rupress.org/jem/article-standard/218/8/e20200938/212491/Reversal-of-emphysema-by-restoration-of-pulmonary

Damage-associated transient progenitor in #AlveolarRegeneration

Lung Organoid+ multi reporter🐭->
Lineage trajectory of AT2 cells of bleomycin lung injury🤠

ILβ-primed AT2->
Cldn4+Ndrg1+Krt8+Ly6a+ bipotent DATP->
AT1

Dr. Joo-Hyeon LeeLab @CellStemCell 2020
https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30287-3

ATF3+ lung capillary #EndothelialCell proliferates & promotes #AlveolarRegeneration post H1N1 injury #Flu

🫁ATF3 KO EC 14 dpi->
⏬Proliferation
⏫Apoptosis
Altered Angiogcrine🤠🧐 to⏬AT1+AT2 epithelial cells

Dr. Edward E. Morrisey lab bioRxiv 2022
https://www.biorxiv.org/content/10.1101/2022.10.14.512212v1.full

Reminiscent of the really cool work from Dr. Katrien De Bock @cell_metabolism 2021

#SkeletalMuscle ATF3/4+ Capillary #EndothelialCell, preferably surrounding oxidative myofibers, as a major cellular origin of Exercise-induced #Angiogenesis😎

https://www.sciencedirect.com/science/article/pii/S1550413121003314

Spatial Atlas of human Lung

Spatially-resolved multi-omics-> #AirwaySubmucosalGland-associated Lymphoid niche for IgA #PlasmaCell + T cells via CCL28/IL6/APRIL paracrine

5 locations, 13 patients

Systemic vs Pulmonary vascular trees

Immune recruiting venous NG2-ABCC9+ICAM1+ #Pericyte is abundant in CCL2/19/21 & CXCL12🤠

Love to dig into transcriptomes of systemic vs pulmonary #SmoothMuscleCell & pericytes along the bronchial tree😋

A spatially resolved atlas of the human lung characterizes a gland-associated immune niche

Love this powerful molecular micro-anatomy for human Lung👍

A great starting point for my Holiday-specific continued biomedical education on Lung🎄

Dr. Sarah Teichmann & Kerstin Meyer lab Nature Genetics 2022
https://www.nature.com/articles/s41588-022-01243-4

Bioelectrical initiation of HeartBeat👹

"a noisy saddle-node on invariant circle bifurcation as the critical phase transition that starts the heart"

A wild & cool piece from Dr Sean Megason & Adam Cohen labs bioRxiv 2022 @SeanMegason @AdamEzraCohen
https://www.biorxiv.org/content/10.1101/2022.12.06.519309v1.full

Also do not miss this great thread from 1st Author @BillZJia
https://twitter.com/BillZJia/status/1601193364451250176

Tumor spheroid-microvasculature-on-a-chip

#Microfluidic
0.6×0.3×25 mm
5 & 15 dyn/cm2
MCF-7 spheroid
HUVEC monolayer

Tumor spheroid alters #EndothelialCell polarization to shear🤠

Sheared EC⏬spheroid growth🤠🤠

Dr. Hongxu Lu & Lining Arnold Ju labs bioRxiv 2022
https://www.biorxiv.org/content/10.1101/2022.11.19.517181v1.full

Vascular #BehçetSyndrome: from pathogenesis to treatment

Superficial & deep vein Thrombosis in 50% patients

Arterial involvement
Thrombosis+Aneurysm of abdominal aorta, pulmonary artery, peripheral arteries

Wait for more human Genetics for this still-mysterious disease 🧐

Dr. Giacomo Emmi lab Nat Rev Rheumatol 2022
https://www.nature.com/articles/s41584-022-00880-7

5-channel (separated by microposts) #Microfluidic for #EndothelialCell-#Pericyte fibrin-
collagen co-culture

Human Lung fibroblast as feeder

Enable study of Synergy b/w #ShearStress & Pericyte on #SproutingAngiogenesis😁

Dr. Ryuji Yokokawa lab, Lab on a chip 2022

Innervation of #PosteriorTibialArtery by branching #TibialNerve

72 calves from 12♀️+24♂️donors

An anatomical basis for improving sympathectomy strategy to treat #ChronicLimbThreatheningIschemia

Dr. Yun Xie & Xiao-Zhen Zhao labs Clin Anat 2022
https://onlinelibrary.wiley.com/doi/abs/10.1002/ca.23997