Thrilled to share our lab's first paper in which we establish genome-wide CRISPR screening within a single mouse! We are so excited about the power of this platform to enable high-throughput genetic dissection of diverse phenomena within a living mammal.
https://www.cell.com/cell-genomics/fulltext/S2666-979X(22)00170-7
#Senescence #ImmuneSurveillance
Next-gen #Senolytic
#ImmuneCheckpointBlockade activates CD8+ T cytotoxicity against (inflammed🔥) PD-L1+ senescent cells->
⏬adverse phenotypes in🐭#Aging+#NASH
Dr. Yoshikazu Johmura & Makoto Nakanishi labs Nature 2022
https://www.nature.com/articles/s41586-022-05388-4
@ondinecleaver2022 @DenisDuboule @opourquie @mads100tist @RogersLabUCD @HaoYin @scottbarolo @kkroll @SpagnoliLab @Mill_lab @jamesbriscoe @MokalledLab @AaronJohnson @ebardot @MarieKmita
Few additions if not overlapping:
@DavidBrueckner (who actually helped many of us to join mastodon!)
@edouardhannezo
@MikiEbisuya
And @knoepfler is here, and was looking for exactly this kind of thread the other day
~50% Olig2+ #Astrocyte Progenitors migrate along blood vessel in prenatal🧠cortex🤠
Vessel-guided migration favors superficial cortex as Astrocyte destination
#EndothelialCell CXCL12-> AP CXCR4/7
Dr. Hidenori Tabata & Kazunori Nakajima labs Nat Comm 2022
https://www.nature.com/articles/s41467-022-34184-x
A new blue fluorescent protein 'Surime' is reported in this paper: "Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application"
https://www.nature.com/articles/s42003-022-04153-7
Pulmonary #ArteryOnAChip
Hypoxia
6 dyn/cm2
Patient ECFC+HPASMC co-culture
#EndothelialCell BMPR2-Sox17->
Prostacyclin production->
⏬#SmoothMuscleCell proliferation
Comparative transcriptomics vs PAH gene sets
Dr. Beata Wojciak-Stothard lab Communications Biology 2022
https://www.nature.com/articles/s42003-022-04169-z
Thank you @ondinecleaver2022 for the initiative
a few more:
@HaoYin @guijacquemet
@dgaboriau
@pj_saez
@rburdine
@juanonyme
@a_tsakiridis @maxvcg @harryleitch @AxelVisel @MHVerlhac @juliettemathieu @t_rayon @bbruneau @Etche_homo @albertcardona
@Amayran
@generegulation
@Sophie_Chauvet @kkroll @Mill_lab @MarieKmita
Everyone who wants to be known by the community should make a post with #DevBio or #DevBiol hashtags.
These will be seen across servers, making it easier to find each other
Antigen-presenting #Pericyte #MultipleSclerosis
PDGFRβ+ Pericyte MHCII KO->
In vitro
⏬CD4+ T cell proliferation & activation
In🐭adoptive transfer EAE
⏫CD3+ T cell entry
No change in BBB, CD4+ T cell, Mɸ, cytokine
Dr. Luisa Klotz lab @IJMS_MDPI 2022
https://www.mdpi.com/1422-0067/23/21/13081/htm
#introduction Hi everyone, I am a developmental biologist, working at the IBDM (Developmental Biology Institute of Marseille) (CNRS and Aix Marseille University). I study muscle development and regeneration, trying to decipher the dysfunctional mechanisms underlying muscle pathologies. I would post with any new equivalent of the very useful #MyoTwitter
Landmark re-read😋
#EndothelialCell #ClonalExpansion in the development of #CerebralCavernousMalformations
"Cavernomas are composed of clones of Ccm3−/− cells that attract Ccm3+/+ cells"
Dr. Matteo Malinverno & Elisabetta Dejana lab Nature Comms 2019
https://www.nature.com/articles/s41467-019-10707-x
Let’s start a thread with all our: #DevBio #DevBiol #science peeps. Please add more below to help us find everybody! Who are your DevBio friends? We need to post to make this place more vibrant:
@DenisDuboule
@opourquie
@mads100tist
@RogersLabUCD
@HaoYin
@scottbarolo
@kkroll
@SpagnoliLab
@Mill_lab
@jamesbriscoe
@MokalledLab
@AaronJohnson
@ebardot
@ondinecleaver2022 @DenisDuboule @opourquie @mads100tist @RogersLabUCD @HaoYin @scottbarolo @kkroll @SpagnoliLab @jamesbriscoe @MokalledLab @AaronJohnson @ebardot
Most excellent community wrangling Ondine! Little trick to filter is to make a #DevBio list- it is a little cumbersome as everyone and their instance needs to be individually searched and added, but then if you click on your list- posts only from those DB 'Mamoots' will appear. It can help focus from the other noise.
@ondinecleaver2022 @DenisDuboule @opourquie @RogersLabUCD @HaoYin @scottbarolo @kkroll @SpagnoliLab @Mill_lab @jamesbriscoe @MokalledLab @AaronJohnson @ebardot Ondine, we could pull the followers and followed by the Twitter SDB account and have it as a CSV file people can use to merge their follows. What do you think?
Isolation of Popliteal Lymphatic Vessels from mouse hindlimb & primarily culture >10k lymphatic mural/muscle cells out of one single vessel🤠
Inject some Evans blue to visualize PLVs
Dr. Guillermo Arroyo-Ataz & Dennis Jones STAR Protocols 2022
https://www.sciencedirect.com/science/article/pii/S2666166722006803
A cancer cell expressing the #Scarlet red fluorescent protein to label the endoplasmic reticulum (a tubular membrane structure inside of cells involved in protein synthesis and secretion) .#FluorescenceFriday
Cardiovascular biologist, Dr. J. Geoffrey Pickering lab, Robarts Research Institute, Western University, London, Ontario, Canada
https://scholar.google.ca/citations?hl=en&user=IbrDx00AAAAJ&view_op