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Thrilled to share our lab's first paper in which we establish genome-wide CRISPR screening within a single mouse! We are so excited about the power of this platform to enable high-throughput genetic dissection of diverse phenomena within a living mammal.

cell.com/cell-genomics/fulltex


Next-gen

activates CD8+ T cytotoxicity against (inflammed🔥) PD-L1+ senescent cells->
⏬adverse phenotypes in🐭+

Dr. Yoshikazu Johmura & Makoto Nakanishi labs Nature 2022
nature.com/articles/s41586-022

Hao Yin boosted

~50% Olig2+ Progenitors migrate along blood vessel in prenatal🧠cortex🤠

Vessel-guided migration favors superficial cortex as Astrocyte destination

CXCL12-> AP CXCR4/7

Dr. Hidenori Tabata & Kazunori Nakajima labs Nat Comm 2022
nature.com/articles/s41467-022

Hao Yin boosted

Great to have an #edit function in #Mastodon, so I could correct a typo (the cross-posted tweet still has the error)

Hao Yin boosted

A new blue fluorescent protein 'Surime' is reported in this paper: "Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application"
nature.com/articles/s42003-022

Pulmonary

Hypoxia
6 dyn/cm2
Patient ECFC+HPASMC co-culture

BMPR2-Sox17->
Prostacyclin production->
proliferation

Comparative transcriptomics vs PAH gene sets

Dr. Beata Wojciak-Stothard lab Communications Biology 2022
nature.com/articles/s42003-022

Hao Yin boosted
Hao Yin boosted

35.5 mile bike today 🚴‍♀️🚴🚴‍♂️Not bad!

Antigen-presenting

PDGFRβ+ Pericyte MHCII KO->
In vitro
⏬CD4+ T cell proliferation & activation

In🐭adoptive transfer EAE
⏫CD3+ T cell entry
No change in BBB, CD4+ T cell, Mɸ, cytokine

Dr. Luisa Klotz lab @IJMS_MDPI 2022
mdpi.com/1422-0067/23/21/13081

Hao Yin boosted

#introduction Hi everyone, I am a developmental biologist, working at the IBDM (Developmental Biology Institute of Marseille) (CNRS and Aix Marseille University). I study muscle development and regeneration, trying to decipher the dysfunctional mechanisms underlying muscle pathologies. I would post with any new equivalent of the very useful #MyoTwitter

Landmark re-read😋

in the development of

"Cavernomas are composed of clones of Ccm3−/− cells that attract Ccm3+/+ cells"

Dr. Matteo Malinverno & Elisabetta Dejana lab Nature Comms 2019
nature.com/articles/s41467-019

Hao Yin boosted

Let’s start a thread with all our: #DevBio #DevBiol #science peeps. Please add more below to help us find everybody! Who are your DevBio friends? We need to post to make this place more vibrant:
@DenisDuboule
@opourquie
@mads100tist
@RogersLabUCD
@HaoYin
@scottbarolo
@kkroll
@SpagnoliLab
@Mill_lab
@jamesbriscoe
@MokalledLab
@AaronJohnson
@ebardot

Hao Yin boosted

Want Mastodon to become more interesting?
1. Connect with other #DevBio #DevBiol peeps here.
2. Post. It’s up to us to start talking.
3. Tell us about who we should follow (not all accounts visible on the follow lists of others, if on different servers).

Hao Yin boosted

@ondinecleaver2022 @DenisDuboule @opourquie @mads100tist @RogersLabUCD @HaoYin @scottbarolo @kkroll @SpagnoliLab @jamesbriscoe @MokalledLab @AaronJohnson @ebardot
Most excellent :ld: :lb: community wrangling Ondine! Little trick to filter is to make a #DevBio list- it is a little cumbersome as everyone and their instance needs to be individually searched and added, but then if you click on your list- posts only from those DB 'Mamoots' will appear. It can help focus from the other noise.

Hao Yin boosted

@ondinecleaver2022 @DenisDuboule @opourquie @RogersLabUCD @HaoYin @scottbarolo @kkroll @SpagnoliLab @Mill_lab @jamesbriscoe @MokalledLab @AaronJohnson @ebardot Ondine, we could pull the followers and followed by the Twitter SDB account and have it as a CSV file people can use to merge their follows. What do you think?

Isolation of Popliteal Lymphatic Vessels from mouse hindlimb & primarily culture >10k lymphatic mural/muscle cells out of one single vessel🤠

Inject some Evans blue to visualize PLVs

Dr. Guillermo Arroyo-Ataz & Dennis Jones STAR Protocols 2022
sciencedirect.com/science/arti

Hao Yin boosted

A cancer cell expressing the #Scarlet red fluorescent protein to label the endoplasmic reticulum (a tubular membrane structure inside of cells involved in protein synthesis and secretion) .#FluorescenceFriday

Hao Yin boosted

Well, sad for my state, happy for some other states, thrilled for my country. Lots of work ahead.

Hao Yin boosted
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