Hao Yin @HaoYin@qoto.org

In #BicuspidAorticValve #Aortopathy, #Aorta-enriched plasma cell-free DNA methylation correlated with ascending aortic #Apoptosis in regions of elevated #WallShearStress (4D flow MRI), but Not aortic diameter

An interesting method to identify tissue-enriched DNA methylation in this paper

Roadmap Epigenomics
BluePrint Epigenome
Metilene
https://www.bioinf.uni-leipzig.de/Software/metilene/

24 candidate aorta-enriched differentially methylated regions
4 used in this study

Dr. Steven Greenway lab Clin Epigenetics 2021
https://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/s13148-021-01137-y

Splendid #SciArt by Kristy Red-Horse lab

Endocardial Origin of Coronary #EndothelialCell +Arteriogenesis

A fascinating BMP2+ transitioning e17.5 coronary EC population

How EC & nonvascular BMPs coordinate heart #Angiogenesis😆

Exogenous BMP2 synergizes with VEGF-A⏫endocardial-derived Angiogenic sprouting from e10.5🫀ventricle explants

LDN193189, BMPR inhibitor,⏬endocardial contribution to coronary vascularization

AAV9-cTnt-Bmp2⏫endocardial-derived angiogenesis in neonatal #MyocardialInfarction🤠

Dev Cell 2022 @kristyredhorse
https://www.cell.com/developmental-cell/fulltext/S1534-5807(22)00759-6

ApoE KO🐶♂️normal diet 18 mo
Multi-arterial #Atherosclerosis->
MI/Stroke/Gangrene

scRNAseq athero-prone vs -resistant arteries
#TenascinC+ #SmoothMuscleCell - LTBP1+ myofibroblast transition in atherogenesis

Dr. X. Wang, YB Zhang, X Ji labs Aging Dis 2022
http://www.aginganddisease.org/EN/10.14336/AD.2022.0313

#Sirt6 control of #EndothelialCell #FattyAcid transport links #Diabetes microvascular dysfunction with #HeartFailurewithPreservedEjectionFraction #HFpEF

EC Sirt6->
⏬H3K9ac PPARγ promoter
⏬CD36,FABP4/5
⏬🐭 Heart Lipid

Dr. Zheng Gen Jin lab Circ Res 2022
https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.121.318988

#Autophagy in🐭#LimbIschemia 1/2

AMPK or Sestrin as potential autophagic regulators

Dr. Mattia Scalabrin & Scott Bowen lab AJP CellPhys 2022
https://journals.physiology.org/doi/abs/10.1152/ajpcell.00174.2022

Which tissue does autophagy occur in (likely #SkeletalMuscle based on Western blot)? And its functional impacts?

#Autophagy in🐭#LimbIschemia 2/2

Evidence on autophagy induced by ischemia/#Hypoxia in #EndothelialCell, and its stimulatory effects on #Angiogenesis

Dr. Joo-Won Jeong lab Cell Death Dis 2020
https://www.nature.com/articles/s41419-020-02849-4

ICAM1-hi #EndothelialCell form Hotspots for #Neutrophil #TransEndothelialMigration (Paracellular)

Blunting ICAM1 heterogeneity
⏬TEM hotspots
⏫TEM-associated endothelial Permeability

Dr. Jaap van Buul lab @emboreports 2022 @joachimgoedhart @VanBuul_lab
https://www.embopress.org/doi/full/10.15252/embr.202255483

Impossible to make a better presentation on this great paper than first-author Max Grönloh @GronlohMax 👇

Also don't miss the informative Discussion session synthesizing the signaling networks of leukocyte TEM

Congratulations!👏👏👏

https://twitter.com/GronlohMax/status/1593299324192047110

Apold1, a #EndothelialCell-enriched gene, in pathological #Angiogenesis
#LimbIschemia ⏬Reperfusion (3 dpi🤓)-> An arteriogenic defect?
#Stroke ⏫border zone vascularization at day 21 but not day 7
Tumor ⏫Nonproductive angiogenesis-> more non-perfusable endothelial channels

Apold1 KO⏬EC Proliferation-How😁

@bohaceklab@nerdculture.de & Katrien de Bock bioRxiv 2022
https://www.biorxiv.org/content/10.1101/2022.12.02.518829v1

Also very interesting, Apold1 KO->⏫KLF2 & KLF4 in EC

Apold1 is recently identified as an ascending aortic EC-enriched gene (vs descending aorta/carotid artery)
Dr. Thomas Quertermous lab bioXRiv 2022
https://www.biorxiv.org/content/10.1101/2022.05.18.492517v1.full

Are Apold1 KO mice ready for #Atherosclerosis/#Aneurysm?

Venous #EndothelialCell as #Angiogenesis origin in🐭#EAE #MultipleSclerosis

Blocking VEGFA (Bevacizumab 7 dpi)
⏬Aberrant angiogenesis
⏫Myelination+neuromotor
BUT w/o altering Immune cell infiltration or Endothelial Barrier (IgG)😎
Dr. Dritan Agalliu lab bioXRiv 2022
https://www.biorxiv.org/content/10.1101/2022.11.15.516660v1

The amoeboid state as part of the epithelial-to-mesenchymal transition programme #EMT

Linking cell Shape control, Cytokine signaling with Cancer Stemness & Malignancy🤠

Dr. Victoria Sanz-Moreno lab @TrendsCellBio 2022
https://www.cell.com/trends/cell-biology/fulltext/S0962-8924(21)00206-3

#Photosynthesis in🐭#Chondrocyte👹

#Biocamouflage
-Use target cell membrane to encapsulate🍃NanoThylakoid Units & evade cross-species elimination
-Supply ATP+NADPH for days in recipient cells

Intra-articular injection🐭causes no overt #ForeignBodyReaction🥸

Metabolic profiles of Chondrocytes+Thylakoids🤠

Dr. Ruikang Tang, Shunwu Fan & Xianfeng Lin labs Nature 2022
https://www.nature.com/articles/s41586-022-05499-y

Photosynthetic medicine for #Osteoarthritis

A lay summary👇
Plant-cell machinery for making metabolites transferred to mammalian cells
https://www.nature.com/articles/d41586-022-03629-0

Cell Encapsulator made as PLLA/PLGA (50:50) electrospun porous (750 nm) scaffold

⏫👤Cardiac mesenchymal cell survival (1 mo in vivo) & #Exosome secretion

Implanted locally->⏫🐭#Limbischemia & #MyocardialInfarction

Dr. David Lundy lab J Controlled Release 2022
https://www.sciencedirect.com/science/article/pii/S0168365922007350

SM22+ #SmoothMuscleCell LRP1 KO->

Spontaneous #SuperiorMesentericArtery Aneurysm

Baseline+Injured carotid arterial defect
(Intensive neointimal Elastin deposits!😈)

⏫Angiotensin+TGFβ signals

Dr. Alan Daugherty & Dudley Strickland labs @JCI_insight 2022
https://insight.jci.org/articles/view/164751

There is a long research history on LRP1 with SMC & arterial wall integrity, starting with this👇

LRP: Role in Vascular Wall Integrity and Protection from Atherosclerosis

Dr. Joachim Herz lab Science 2003
https://www.science.org/doi/10.1126/science.1082095