Julien Delpierre @JulienDelpierre@qoto.org

I moved back to #paris !
Starting a #postdoc with the Bellaïche lab at the @institut_curie (Sorry I brought the German weather with me.)
I'm so glad to work with #Drosophila again.

Metrics reloaded. "A comprehensive framework guiding researchers in the problem-aware selection of metrics."
A must-read for everyone doing #ImageAnalysis in #biology
https://arxiv.org/abs/2206.01653

A fly library of fluorescently labeled RhoGEF/GAP to unveil molecular mechanisms of de novo junction formation and neighbor response upon cell division.
#Drosophila #development #biology #CellDivision #MechanoBiology #MechanoSensing
https://www.cell.com/current-biology/fulltext/S0960-9822(23)00062-3

"[...] a tension-dependent signal on the #nucleus that sets the time for nuclear envelope permeabilization (NEP) and mitotic entry. This signal relies on #actomyosin #contractility, which unfolds the nucleus during the G2-M transition, activating the stretch-sensitive #cPLA2 on the nuclear envelope and regulating the nuclear translocation of #cyclin B1.[...]"
https://rupress.org/jcb/article-abstract/221/12/e202205051/213539/Nuclear-tension-controls-mitotic-entry-by
#BioMechanics #MechanoBiology #NuclearTension #CellBiology #CellCycle #CellDivision #NuclearEnvelope #NuclearTranslocation #CellCompression #GenomicInstability

"[...] varying the duration of #BMP #signaling leads to either pluripotent, mesodermal, and extraembryonic states, while varying the concentration does not cause efficient mesodermal differentiation at any dose [...] appropriately timed pulse of BMP induces #hPSCs to a mesodermal fate more efficiently that sustained signaling at any concentration. [...] this effect is due to a combinatorial interpretation of the applied BMP signal and induced endogenous #Wnt signaling."
https://www.biorxiv.org/content/10.1101/2022.11.11.516212v1?rss=1
#DevBio #CellFate #GermLayers #morphogens #Differentiation

I came across this #microscopy paper. https://www.nature.com/articles/s41587-022-01519-4
"By blue-shifting the #STED beam and separating #fluorophores by on/off switching, individual fluorophores bound to a #DNA strand are localized with σ = 4.7 Å, corresponding to a fraction of the fluorophore size, with only 2,000 detected #photons ."
This image showing resolution below the size of the fluorophore leaves me speechless.
The paper features images of #DNAorigami, #NuclearPores, synaptic #Vesicles, #ECM (#laminin) and #vilmentin

Beautiful blog post reviewing the cognitive capacity of multiple animal species. Their work focuses on #animalwelfare and #cognition. #cognitivescience #behavior #physiology
https://forum.effectivealtruism.org/posts/tnSg6o7crcHFLc395/the-welfare-range-table