Dan Mucida boosted

'The presence of arsenic is not uncommon in antique artifacts. The element is prevalently found in green pigments that were once used to dye clothing, book covers and even artificial flowers, according to Ms. Ritchie. (In the Victorian era, she said, people even ate small amounts of the toxin, hoping to make their skin appear pale.)'
HT @mucida

nytimes.com/2023/09/23/science

Dan Mucida boosted

"We find that purchases dominate laboratory emissions, accounting for more than 50% of emissions, with a median of 2.7 t CO2e/pers, which is 3 to 4-fold the separate contribution from travel, commutes and heating"

cc @mucida

biorxiv.org/content/10.1101/20

Purchases dominate the carbon footprint of research laboratories

Despite increasing interest for the carbon footprint of higher education institutions, little is known about the carbon footprint associated to research activities. Air travel and attendance to conferences concentrate recent data and debates but purchases have attracted little attention. Here we develop a hybrid method to estimate the greenhouse gas emissions (GHG) associated to research purchases. To do so, we combine macroe-conomic databases, research-centered companies footprints and life-cycle assesments to construct a public database of monetary emission factors (EF) for research purchases. We apply it to estimate the purchases emissions of a hundred of research laboratories in France, belonging to the Labos 1point5 network and gathering more than 20000 staff, from all disciplines. We find that purchases dominate laboratory emissions, accounting for more than 50% of emissions, with a median of 2.7 t CO2e/pers, which is 3 to 4-fold the separate contribution from travel, commutes and heating. Median electricity emissions are 5-fold lower in our dataset of laboratories using low carbon electricity but they become preponderant for high carbon electricity mixes (3.5 t CO2e/pers). Purchases emissions are very heterogeneous among laboratories and are linearly correlated with budget, with an average carbon intensity of 0.31 ± 0.07 kg CO2e/€ and differences between research domains. Finally, we quantify the effect of a series of demand-driven mitigation strategies obtaining up to − 20 % in total emissions ( − 40 % in purchases emissions), suggesting that effectively reducing the carbon footprint of research activities calls for systemic changes. Significance statement Research activities are recently interrogating their contribution to global warming, mainly through the impact of air travel but neglecting the emissions embodied in scientific purchases. However, goods and services used in a research laboratory emit greenhouse gases when they are produced. Here we construct a public and robust database of emission factors to quantify purchases emissions in a laboratory and we use it to assess emissions from a hundred of laboratories in France, from all disciplines. We find that purchases emissions represent half of the of the 6.3 t CO2e/pers per year emitted on average per laboratory. Emissions, however, vary greatly between laboratories and disciplines and an analysis of mitigation strategies shows that decreasing demand may significantly reduce purchases emissions. ### Competing Interest Statement The authors have declared no competing interest.

www.biorxiv.org
Dan Mucida boosted

"The effects of climate change driven by human activity are now part of the daily news cycle, and time is running out for decisive action."

Lab & academia's responsibilities in the climate emergency. A privilege to collaborate with @mucida & Rachel on this Nature Immunology commentary.

nature.com/articles/s41590-023

Dan Mucida boosted

A reference framework to normalize human lymphocyte numbers in ageing.

'In this study, we analyze cross-sectional numbers of mainly T lymphocytes (CD3+, CD3+CD4+, and CD3+CD8+) and their subpopulations (naive and memory) from 673 healthy Dutch individuals ranging from infancy to adulthood (0–62 y). We fitted the data by a delayed exponential function and estimated parameters for each lymphocyte subset.'

journals.aai.org/jimmunol/arti

Dan Mucida boosted

It was a pleasure to reconnect with José Roberto de Toledo of Brazil's Piauí magazine a couple years after participating in his Portuguese language Pandemic Podcast, "Luz no fim da Quarentena" (ht @mucida).
On UOL's Análise de Notícia we talked about the new and .
👇 Starts around min 27 (in Portuguese)

youtube.com/watch?v=N68508vkQ-

10/ Thanks to everyone involved in this 25 year-old project, particularly Ainsley Lockhart, Aubrey Reed, Tiago Castro, Calvin Herman, Ciça Canesso and all Mucida lab members, particularly Roham Parsa, who developed the fate-mapping model used in collaboration with the Nussenzweig lab.

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9/ These findings provide important insights into the mechanisms underlying the ability of the intestinal immune system to tolerate food antigens, and could have implications for the development of new therapies for food allergies.

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8/ The study also identified both steady-state epithelium-adapted CD4+ T cells and tolerance-induced Tregs that recognize dietary antigens, suggesting that both cell types may be critical for preventing inappropriate immune responses to food.

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7/ Such steady-state CD4+ T cell response to food was disrupted by an inflammatory challenge, and protection against food allergy via previous ingestion of the protein (oral tolerance) was associated with Treg clonal expansion and decreased pro-inflammatory gene expression.

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6/ This tissue specialized transcriptional program includes cytotoxic genes on both conventional and regulatory CD4+ T cells (Tregs), also previously addressed by the lab (science.org/doi/10.1126/scienc, or nature.com/articles/s41590-021)

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5/ These observations inform about the antigen sources of CD4 T cell adaptation to the gut epithelium, topic covered by the lab for over a decade (see nature.com/articles/ni.2518)

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4/ Our study found that dietary proteins contribute to the accumulation and clonal selection of antigen-experienced CD4+ T cells in the intestinal epithelium, imprinting a tissue-specific transcriptional program on both conventional and regulatory CD4+ T cells.

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3) Our new study used antigenically defined diets and gnotobiotic models to find individual contributions from food versus microbiota on the profile and T cell receptor repertoire of intestinal CD4+ T cells.

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2/ Ainsley Lockhart, who recently brilliantly defended her thesis, took on this question and answered more comprehensively that I could ever dream when I started working on this question exactly 25 years ago.

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1/ What is the physiological impact of dietary proteins on steady-state T cells? Nelson Vaz and Ana Faria, my undergrad mentors, wondered about this question for decades.

biorxiv.org/content/10.1101/20

Dietary protein shapes the profile and repertoire of intestinal CD4+ T cells

The intestinal immune system must tolerate food antigens to avoid allergy, a process requiring CD4+ T cells. Combining antigenically defined diets with gnotobiotic models, we show that food and microbiota distinctly influence the profile and T cell receptor repertoire of intestinal CD4+ T cells. Independent of the microbiota, dietary proteins contributed to accumulation and clonal selection of antigen-experienced CD4+ T cells at the intestinal epithelium, imprinting a tissue specialized transcriptional program including cytotoxic genes on both conventional and regulatory CD4+ T cells (Tregs). This steady state CD4+ T cell response to food was disrupted by inflammatory challenge, and protection against food allergy in this context was associated with Treg clonal expansion and decreased pro-inflammatory gene expression. Finally, we identified both steady state epithelium-adapted CD4+ T cells and tolerance-induced Tregs that recognize dietary antigens, suggesting that both cell types may be critical for preventing inappropriate immune responses to food. ### Competing Interest Statement The authors have declared no competing interest.

www.biorxiv.org
Dan Mucida boosted

A new preprint from Ainsley Lockhart & @mucida has, as the cool kids say, just dropped:

"Dietary protein shapes the profile and repertoire of intestinal CD4+ T cells"

biorxiv.org/content/10.1101/20

Dan Mucida boosted

Some of my favorite cover illustrations by Madalena were not selected by editors - like this one for Ilana Gabanyi & @mucida's 2016 paper "Neuro-immune Interactions Drive Tissue Programming in Intestinal Macrophages"

cell.com/cell/fulltext/S0092-8

madalenaparreira.com

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