Dear fellow 🔬
whether you work in , or ,
we, the (part DGE dge-homepage.de) and BerlinUAlliance invite you to our Electron Microscopy Spring School 2024.
Thread ahead 🧵

The school will take place from Sunday 03 March 2024 to Wednesday 06 March 2024.

In short, the school offers EM-lectures 👩‍🏫👨‍🏫, hands-on courses👨‍🔬👩‍🔬 and data analysis👩‍💻👨‍💻
The first day gives you the chance to present your work🥳 and to get in contact with your peers🤝

❗️You can view the program here:
ydge.de/em-spring-school-2024/
❗️You can register for the workshop here:
docs.google.com/forms/d/e/1FAI
The participation fee will be 50€ for students and PhD-students and 60€ for non-students.

We thank our sponsors 💯 (in random order) BerlinUAlliance, FU_Berlin, BMBF_Bund, HumboldtUni, Berlin Senatsverwaltung für Wissenschaft, Gesundheit, Pflege und Gleichstellung, TUBerlin, IRISAdlershof, ChariteBerlin, CSMB_HU, Protochips and nion company

If you have further questions 🤔 contact us via contact@ydge.de.
Join our mailing list 📧 to don't miss out any further events of : listserv.dfn.de/sympa/subscrib
Also, we have a discord channel where you can reach yDGE: discord.com/invite/mEEc2dvVcJ

See you soon,

yours yDGE

Very recent paper on an application of HAADF detectors to perform single counting:

nature.com/articles/s41467-023

Our working group within the 🔬has a new team with new and old members. Have a look at our website:
ydge.de

Calling all budding ! 👩‍🔬 👨‍🔬 Don't miss our virtual International on May 7th, 2023 at 6 PM UTC via Zoom. Join us for relaxed conversations, online games, and a chance to win one of three raffle draw prizes sponsored by Thermo Fisher, MAS, and MSA. Register at forms.gle/MK4e7oPhYmuisGTw7. See you there! 🔬

It's the second day of . Especially as an early career scientist, you should not miss our later this evening at 18:15 (6:15 pm) in the room vanadium at darmstadtium.

Ph.D. student and expert talks 🥳

With the Microscopy Conference 2023 approaching, we would like to share ten tips for folks who are going to a conference for the first time.

1) Preparation:
1. Don't worry: A conference can be overwhelming, lots of people, large venue, many parallel sessions. But just observe and experience :)

2. Search the conference agenda for possibilities to connect to other students at Young Scientists events. These can be called student mixers and/or can be organized by the ‘youth association’ of the conference’s parent organization (eg. DGE/yDGE).

3. Look through the program and find out which sessions you definitely don't want to miss.

4. Dress code? It's a scientist's event, but it could be wise to look a bit more professional during your own presentation (sorts of 'smart casual', suits or costumes not needed)

a. If the question really gives you no peace: Check out the photos from past conferences in your field. You can usually get a good feeling on what the ‚dress code consensus‘ is.

5. You will usually walk around a lot: wear comfortable shoes

6. Can happen: venue is overly air-conditioned -> be prepared and bring a sweater or jacket

2) During the conference:
1. Find out where you will get food+beverages (lunchtime lectures, coffee breaks, shops around the venue)

2. Try to connect known names to faces.

3. Poster sessions are especially useful to make contact, and presenters are usually happy if you reach out to them. Simply saying that you're new to the field and asking if they can briefly say what their work is about is OK!

4. Remember: You are not the only first-timer at the conference!

🌐 📝

In addition, we would like to advertise our yDGE Symposium:

It will take place on 28/02/2023 from 18:15 until 20:15 in the Vanadium room.

Right after, we will also host a Social Networking Session. We are very much looking forward to connect with you in person in Darmstadt.

Do you have additional tips for conference newcomers? Please share them below!

Want to work at the intersection of morphometry and representation learning for bioimage analysis?

We have TWO open postdoctoral positions! Each comes with its own challenging biological question, unique dataset, and exceptional team of collaborators!

Details below👇

The identity of extra densities in #cryoEM maps of #amyloids from brains remains unknown, and a major focus of research. Just because a model fits in these fuzzy densities does not mean much, and writing speculative papers is going to be of little help.
biorxiv.org/content/10.1101/20

RNA as a component of fibrils from Alzheimer’s disease and other neurodegenerations

Fibrils from brains of patients with Alzheimer’s disease[1][1]–[5][2], Parkinson’s disease[6][3], amyotrophic lateral sclerosis[7][4] and other neurodegenerations[3][5],[4][6],[8][7]–[18][8] contain unknown molecules. Extra densities (EDs), containing these unknown molecules, are available to examine in electron cryo-microscopy maps from the Electron Microscopy Data Bank[19][9], a public repository. EDs can be visualised in their protein environments using matched atomic models from the Protein Data Bank[20][10], another public repository. Lysine-coordinating EDs from a wide range of neurodegenerative diseases[1][1]–[6][3],[8][7]–[18][8] and EDs from the glycine-rich region of TAR DNA-binding protein 43 (TDP-43) fibrils in amyotrophic lateral sclerosis with frontotemporal lobar degeneration (ALS-FTLD)[7][4] were the subject of the present study. EDs ran parallel to the fibril axis and at right angles to protein with a repeat distance matching that of protein. They formed connections with protein consistent with a role in the guided assembly of fibrils. They had a connectivity pattern and estimated molecular weights consistent with ribonucleic acid (RNA). A straight form of RNA (ortho-RNA, oRNA) was modelled into one ED. It fitted other EDs and formed a rich symmetrical network of hydrogen bonds when docked to protein, implicating RNA as a unifying and organising factor in neurodegeneration. A new hypothesis of neurodegeneration (ponc, protein ortho-nucleic acid complex, pronounced ponk) is proposed in which RNA is the driver of these diseases. According to the ponc hypothesis, a particular RNA sequence (likely repetitive) enciphers a particular strain of ponc agent with its own protein fold and type of neurodegeneration. Ponc provides an explanation of fibril growth and replication, species barrier and adaptation, inherited neurodegeneration, resistance to chemicals and irradiation, protein-free transmission and co-pathologies. Ponc may also be relevant to other chronic diseases and origins of life. New treatments might be possible, targeting the unique chemical and physical properties of ponc. ### Competing Interest Statement The authors have declared no competing interest. [1]: #ref-1 [2]: #ref-5 [3]: #ref-6 [4]: #ref-7 [5]: #ref-3 [6]: #ref-4 [7]: #ref-8 [8]: #ref-18 [9]: #ref-19 [10]: #ref-20

www.biorxiv.org

A collaborator of mine at TU Wien is looking for a postdoctoral research to join our joint project – fun with ions and and electron microscopy in 2D materials!

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