Hao Yin @HaoYin@qoto.org

#EndothelialCell ARF6 GTPase links IL-1β inflammation with TGFβ/BMP-SMAD1/5 #EndMT

EC Arf6 KO in🐭#EAE->
⏫#BBB #BloodSpinalCordBarrier
⏬T cell infiltration & demyelination
w/o altering normal immunity

Dr. Shannon Odelberg, Weiquan Zhu labs Neuron 2022
https://www.sciencedirect.com/science/article/pii/S0896627322006602

Phospholipase Cε #Aortopathy

Plce1 KO🐭 are susceptible to ANGII-induced ascending #AorticAneurysm+Dissection

Role of adventitial myofibroblast cytokines?😎

Dr. Alan Smrcka, Santhi Ganesh, Markus Bitzer labs AJP Heart Circ 2022
https://journals.physiology.org/doi/abs/10.1152/ajpheart.00262.2022

Kidney epithelial #Tubuloid

CD24+ epithelial progenitor-derived
4-phase protocol, Matrigel +Wnt3a/RSPO1, grow > 6 mo
CRISPR modeling #ADPKD, Cyst size responsive to #Tolvaptan

Super-impressive single-cell or single-nuclei RNAseq analyses to dissect the spatiotemporal molecular/cellular dynamics of this cool renal tubuloid, laying a critical foundation to enhance its clinical utilities👍

Wonderful #Lumenogenesis of CD24+ epithelial progenitor-derived nephrospheres, day 7 in Matrigel🤠

Dr. Rafael Kramann lab @NatureGenet 2022 @jackxuyx @KuppeChristoph @rkramann
https://www.nature.com/articles/s41588-022-01202-z

Editing a gateway for #CellTherapy across #BloodBrainBarrier

An excellent review🤠 Learn a lot!

Lesson from Leukocyte-#BBB interaction->
Engineering strategies->
⏫Cell delivery to injured🧠#Stroke

#openaccess

Dr. Rust Ruslan lab Brain 2022
https://academic.oup.com/brain/advance-article/doi/10.1093/brain/awac393/6832391

#EndothelialCell MEK1 #VascularMalformation

P1 topical 4OHT-induced mosaic EC MEK1 K57N transgene (what is the efficiency🧐)->
50% death by P30 +multiorgan VM (Arteriovenous malformation or other lesions) & Hemorrhage

Lesions composed of both mutant & WT EC

Dr. Patrick Smits lab Angiogenesis 2022
https://link.springer.com/article/10.1007/s10456-022-09853-6

12q, an optimized small chemical activator of #Sirt6
Comprehensive analyses vs MDL-800->
⏫Sirt6 deacetylase & demyristoylase activities
⏫Anti-#PancreaticDuctalAdenoCarcinoma activity

Look forward to more studies on its Non-tumoral benefits?🧐

Dr. Shengyong Yang lab J Med Chem 2022
https://pubs.acs.org/doi/full/10.1021/acs.jmedchem.0c01183

Pancreatic Rab11a/Rab11b dKO->
Aberrant polarized transport of apical/basolateral proteins->
AMIS supernumerary->
Defects in ductal #Lumenogenesis; Acinar morphogenesis; Pancreatic cell lineage dysregulation

Highly intriguing, PancRab11KO->⏬Sox9+ bipotent progenitor & #Islet mass-> #GlucoseIntolerance in suvived adult🐭

Lumenized epithelial paracrine or endocrine-autonomous defect?😆

No baseline change in fast glucose. Insulin/glucagon? Adult islet histology-Amyloid degeneration?

The branched, lumenized pancreatic duct is fragmented into disconnected Microlumens + enlarged Cyst in PancRab11KO🐭

Can't help but think Rab35 in anisotropic Lumenogenesis of liver #BileCanaliculi

Is there a primary hydro-osmotic defect here?😋

Still, what happens to pancreatic vascular morphogenesis in Panc-Rab11 KO🐭?(Surely, epithelia & endothelia are coupled🤪)

More importantly, now it's the magic timing if you also want to have such amazing stories

Cleaver lab is recruiting TWO postdocs

Dr. Ondine Cleaver lab @ondinecleaver2022 bioXRiv 2022
https://www.biorxiv.org/content/10.1101/2022.11.17.516976v1

Airway #SmoothMuscleCell #Asthma Inflammation

SMMHC-driven #Integrin α7 transgene in ovalbumin♀️🐭->
⏬Airway leukocyte infiltration
⏬Pulmonary resistance (not compliance)
Re-balanced cytokine/chemokine production

Dr. Mariam Ba lab FASEB Bioadv 2022
https://faseb.onlinelibrary.wiley.com/doi/10.1096/fba.2022-00050

Some #LongNonCodingRNAs encode functional peptides😎

LncPSR & its protein, Arteridin, complex with Ybx1->
⏬#SmoothMuscleCell contractile phenotype

AAV2-SM22α-shPSR⏬ANGII-induced SMC hypertrophy in🐭aorta

Dr. Y Wang, G Wu, C Zeng labs @CircRes 2022
https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.122.321080

#EZClear😎

50% tetrahydrofuran in ddH2O to clear🐭🧠etc ≤ 48 h
80% Nycodenz+7M urea-> RI 1.518
Post-clearing cryosectionable, whole-mount immunofluorescence
Minimal volume change

Dr Chih-Wei Hsu & Joshua Wythe labs eLife 2022 @CWLoganHsu @wythelab
https://elifesciences.org/articles/77419

Landmark👹

How fetal liver vasculature serves as a transient niche for #HematopoieticStemCell expansion while undergoing #BranchingMorphogenesis

Fetal liver hematopoietic stem cell niches associate with portal vessels

Dr. Paul Frenette lab Science 2015
https://www.science.org/doi/10.1126/science.aad0084

#Senescence #ImmuneSurveillance
Next-gen #Senolytic

#ImmuneCheckpointBlockade activates CD8+ T cytotoxicity against (inflammed🔥) PD-L1+ senescent cells->
⏬adverse phenotypes in🐭#Aging+#NASH

Dr. Yoshikazu Johmura & Makoto Nakanishi labs Nature 2022
https://www.nature.com/articles/s41586-022-05388-4

~50% Olig2+ #Astrocyte Progenitors migrate along blood vessel in prenatal🧠cortex🤠

Vessel-guided migration favors superficial cortex as Astrocyte destination

#EndothelialCell CXCL12-> AP CXCR4/7

Dr. Hidenori Tabata & Kazunori Nakajima labs Nat Comm 2022
https://www.nature.com/articles/s41467-022-34184-x

Pulmonary #ArteryOnAChip

Hypoxia
6 dyn/cm2
Patient ECFC+HPASMC co-culture

#EndothelialCell BMPR2-Sox17->
Prostacyclin production->
⏬#SmoothMuscleCell proliferation

Comparative transcriptomics vs PAH gene sets

Dr. Beata Wojciak-Stothard lab Communications Biology 2022
https://www.nature.com/articles/s42003-022-04169-z

Antigen-presenting #Pericyte #MultipleSclerosis

PDGFRβ+ Pericyte MHCII KO->
In vitro
⏬CD4+ T cell proliferation & activation

In🐭adoptive transfer EAE
⏫CD3+ T cell entry
No change in BBB, CD4+ T cell, Mɸ, cytokine

Dr. Luisa Klotz lab @IJMS_MDPI 2022
https://www.mdpi.com/1422-0067/23/21/13081/htm

Landmark re-read😋

#EndothelialCell #ClonalExpansion in the development of #CerebralCavernousMalformations

"Cavernomas are composed of clones of Ccm3−/− cells that attract Ccm3+/+ cells"

Dr. Matteo Malinverno & Elisabetta Dejana lab Nature Comms 2019
https://www.nature.com/articles/s41467-019-10707-x

Isolation of Popliteal Lymphatic Vessels from mouse hindlimb & primarily culture >10k lymphatic mural/muscle cells out of one single vessel🤠

Inject some Evans blue to visualize PLVs

Dr. Guillermo Arroyo-Ataz & Dennis Jones STAR Protocols 2022
https://www.sciencedirect.com/science/article/pii/S2666166722006803

Surgery or Endovascular Therapy for #ChronicLimbThreateningIschemia

A good-quality great saphenous vein for conduit+
Surgery-first->
32%⏬risk of MALE/Death vs endovascular strategy

Dr. Alik Farber, Matthew Menard, et al BEST_CLI NEJM 2022
https://www.nejm.org/doi/10.1056/NEJMoa2207899

#EndothelialCell Mst1 KO->
⏫EC inflammation by #OscillatoryShearStress 0.5±4 dyn/cm2 1 Hz
⏫🐭#Atherosclerosis

Independent of #YAP (?TAZ🧐)

Mst1 phosphorylates #Connexin43->⏬Hemichannel activity, not gap junction coupling

Dr. Ding Ai lab Circ Res 2022
https://www.ahajournals.org/doi/full/10.1161/CIRCRESAHA.122.321322

Arterial dictation of fetal #Hematopoiesis👹

Fetal bone marrow Bmx+ arterial #EndothelialCell (not Myh11+ mural cell) Wnt2->
⏫c-Kit+ HSPC β-catenin signals->
⏫colonization & transplant engraftment

Dr. Ralf Adams lab Nature Commmnications 2022
https://www.nature.com/articles/s41467-022-28775-x