Hao Yin @HaoYin@qoto.org
Cardiovascular biologist, Dr. J. Geoffrey Pickering lab, Robarts Research Institute, Western University, London, Ontario, Canada
https://scholar.google.ca/citations?hl=en&user=IbrDx00AAAAJ&view_op
Joined Nov 2022
ApoE KO🐶♂️normal diet 18 mo
Multi-arterial #Atherosclerosis->
MI/Stroke/Gangrene
scRNAseq athero-prone vs -resistant arteries
#TenascinC+ #SmoothMuscleCell - LTBP1+ myofibroblast transition in atherogenesis
Dr. X. Wang, YB Zhang, X Ji labs Aging Dis 2022
http://www.aginganddisease.org/EN/10.14336/AD.2022.0313
#Sirt6 control of #EndothelialCell #FattyAcid transport links #Diabetes microvascular dysfunction with #HeartFailurewithPreservedEjectionFraction #HFpEF
EC Sirt6->
⏬H3K9ac PPARγ promoter
⏬CD36,FABP4/5
⏬🐭 Heart Lipid
Dr. Zheng Gen Jin lab Circ Res 2022
https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.121.318988
#Autophagy in🐭#LimbIschemia 1/2
AMPK or Sestrin as potential autophagic regulators
Dr. Mattia Scalabrin & Scott Bowen lab AJP CellPhys 2022
https://journals.physiology.org/doi/abs/10.1152/ajpcell.00174.2022
Which tissue does autophagy occur in (likely #SkeletalMuscle based on Western blot)? And its functional impacts?
#Autophagy in🐭#LimbIschemia 2/2
Evidence on autophagy induced by ischemia/#Hypoxia in #EndothelialCell, and its stimulatory effects on #Angiogenesis
Dr. Joo-Won Jeong lab Cell Death Dis 2020
https://www.nature.com/articles/s41419-020-02849-4
ICAM1-hi #EndothelialCell form Hotspots for #Neutrophil #TransEndothelialMigration (Paracellular)
Blunting ICAM1 heterogeneity
⏬TEM hotspots
⏫TEM-associated endothelial Permeability
Dr. Jaap van Buul lab @emboreports 2022 @joachimgoedhart @VanBuul_lab
https://www.embopress.org/doi/full/10.15252/embr.202255483
Impossible to make a better presentation on this great paper than first-author Max Grönloh @GronlohMax 👇
Also don't miss the informative Discussion session synthesizing the signaling networks of leukocyte TEM
Congratulations!👏👏👏
Apold1, a #EndothelialCell-enriched gene, in pathological #Angiogenesis
#LimbIschemia ⏬Reperfusion (3 dpi🤓)-> An arteriogenic defect?
#Stroke ⏫border zone vascularization at day 21 but not day 7
Tumor ⏫Nonproductive angiogenesis-> more non-perfusable endothelial channels
Apold1 KO⏬EC Proliferation-How😁
@bohaceklab@nerdculture.de & Katrien de Bock bioRxiv 2022
https://www.biorxiv.org/content/10.1101/2022.12.02.518829v1
Also very interesting, Apold1 KO->⏫KLF2 & KLF4 in EC
Apold1 is recently identified as an ascending aortic EC-enriched gene (vs descending aorta/carotid artery)
Dr. Thomas Quertermous lab bioXRiv 2022
https://www.biorxiv.org/content/10.1101/2022.05.18.492517v1.full
Are Apold1 KO mice ready for #Atherosclerosis/#Aneurysm?
Venous #EndothelialCell as #Angiogenesis origin in🐭#EAE #MultipleSclerosis
Blocking VEGFA (Bevacizumab 7 dpi)
⏬Aberrant angiogenesis
⏫Myelination+neuromotor
BUT w/o altering Immune cell infiltration or Endothelial Barrier (IgG)😎
Dr. Dritan Agalliu lab bioXRiv 2022
https://www.biorxiv.org/content/10.1101/2022.11.15.516660v1
The amoeboid state as part of the epithelial-to-mesenchymal transition programme #EMT
Linking cell Shape control, Cytokine signaling with Cancer Stemness & Malignancy🤠
Dr. Victoria Sanz-Moreno lab @TrendsCellBio 2022
https://www.cell.com/trends/cell-biology/fulltext/S0962-8924(21)00206-3
#Photosynthesis in🐭#Chondrocyte👹
#Biocamouflage
-Use target cell membrane to encapsulate🍃NanoThylakoid Units & evade cross-species elimination
-Supply ATP+NADPH for days in recipient cells
Intra-articular injection🐭causes no overt #ForeignBodyReaction🥸
Metabolic profiles of Chondrocytes+Thylakoids🤠
Dr. Ruikang Tang, Shunwu Fan & Xianfeng Lin labs Nature 2022
https://www.nature.com/articles/s41586-022-05499-y
Photosynthetic medicine for #Osteoarthritis
A lay summary👇
Plant-cell machinery for making metabolites transferred to mammalian cells
https://www.nature.com/articles/d41586-022-03629-0
Cell Encapsulator made as PLLA/PLGA (50:50) electrospun porous (750 nm) scaffold
⏫👤Cardiac mesenchymal cell survival (1 mo in vivo) & #Exosome secretion
Implanted locally->⏫🐭#Limbischemia & #MyocardialInfarction
Dr. David Lundy lab J Controlled Release 2022
https://www.sciencedirect.com/science/article/pii/S0168365922007350
SM22+ #SmoothMuscleCell LRP1 KO->
Spontaneous #SuperiorMesentericArtery Aneurysm
Baseline+Injured carotid arterial defect
(Intensive neointimal Elastin deposits!😈)
⏫Angiotensin+TGFβ signals
Dr. Alan Daugherty & Dudley Strickland labs @JCI_insight 2022
https://insight.jci.org/articles/view/164751
There is a long research history on LRP1 with SMC & arterial wall integrity, starting with this👇
LRP: Role in Vascular Wall Integrity and Protection from Atherosclerosis
Dr. Joachim Herz lab Science 2003
https://www.science.org/doi/10.1126/science.1082095
TMEM100, a selective human mouse Lung #EndothelialCell Marker
Higher in Capillary EC & Aerocyte than in arterial/venous EC
TMEM100⏫EC proliferation
Detected in scattered Tmem100+ Lymphatic EC & pericyte
Look forward to Tmem100-CreERT2🐭for Lung diseases😁
#PAH #PulmonaryFibrosis #Emphysema #coronavirus
Dr. Zhiyu Dai lab @atvbahajournals 2022 @joeydai
https://www.ahajournals.org/doi/full/10.1161/ATVBAHA.122.317683
Mine your own endothelial gene of interest here
https://www.ebi.ac.uk/gxa/sc/experiments/E-MTAB-8077/results/tsne
Rictor is an FGF2-responsive gene in #EndothelialCell
Embryonic EC Rictor KO dampens FGF-dependent #Angiogenesis in multi-organs (& delays ossification of digit bones+vertebrae😎), leading to embryonic lethality
Dr. Rok Humar lab @SciReports 2015
https://www.nature.com/articles/srep17705
Rictor, a core mTORC2 component, as an #EndothelialCell Mechanotransducer
EC Rictor KO in young🐭->
⏬Shear (2 or 15 dyn/cm2)-induced HUVEC alignment
⏬VE-cad junction
⏬Low shear-induced vWF
Dr Shao-Liang Chen & Jun-Xia Zhang labs @FrontCellDevBio 2022
https://www.frontiersin.org/articles/10.3389/fcell.2022.963866/full
#Senescence of Post-mitotic #Neuron🤓
Cortex ~1% non-demented Ctrl vs ~3% #AlzheimersDisease
Senescence spectrum of induced Neurons
Electrical defects
pro-astrogliosis SASP
Dr Joseph Herdy, Jerome Mertens & Fred Gage labs @CellStemCell 2022
https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(22)00457-X
10k steps/day physical activity (Garmin) for 6 mo Slow down enlargement rate of #AorticRoot (Echo) in #MarfanSyndrome
15 Ctrl 12 yr+24 Intervention 14 yr
0.13 vs -0.01 cm/yr 🤠
Dr. Elif S.S. Tierney lab JAHA 2022 @MarfanFdn
https://www.ahajournals.org/doi/10.1161/JAHA.122.027598
Can anyone rec papers on the normal growth rate of aorta dimension in healthy children/teenagers?🙏
Look at this gorgeous movie on Neutrophil (Green) talking with #EndothelialCell (Blue), #Pericyte (Magenta) & #MastCell (Cyan) in Venule during #Diapedesis 2 h post-TNFα challenge 🤩
Perivascular #MastCell IL-17A->
⏫MC-proximal venular #Pericyte/Mural cell (not #EndothelialCell) Surface presentation of ICAM1 & CXCL1->
⏫#Neutrophil #Diapedesis in subendothelial space
Dr. Mathieu-Benoit Voisin lab @NatureComms 2022 @RegisJoulia
https://www.nature.com/articles/s41467-022-34695-7
Vascular #SmoothMuscleCell dysfunction in #Neurodegeneration
Vasculo-neuronal-inflammatory triad
Hypercontractile VSMC
⏬Clearance receptors for Aβ
Autoimmune-related SMC degeneration in #AlzheimersDisease
Dr. Genevieve Hayes lab @FrontNeurosci 2022
https://www.frontiersin.org/articles/10.3389/fnins.2022.1010164/full
Amazing developmental biologist🤩
Such a cell contact-tracking system is already taking shape in Drosophila as early as 2016!👍
Dr. Carlos Lois lab @JCellBiol
Monitoring cell-cell contacts in vivo in transgenic animals
Is there an even-earlier example!?😆
A mind-blowing paper from Dr. Bin Zhou lab🤯
Genetically Labeling/Tracing Cell Contact events & post-contact consequences
Tigre-SynNotch + Cre & Dre tri-transgenic🐭
One can track Ongoing+History of Contact (likely minimal 2-h) b/w any 2 cell types
Embryonic Cardiac #EndothelialCell not only constitutes 20% of Liver vessels but also colonizes Skeletal muscle (~5% Fig S11B)
How will this contact tracking system revolutionize our research?😄
Dendritic cell-> atherogenic SMC?
Senescent pericyte->Capillary shear sensing?
#SmoothMuscleCell Piezo1 promotes high Phosphate-induced #Calcification & osteogenic differentiation of cultured👤aortic SMC (& culture🐭aorta)
How Piezo1-Ca2+ elevates expression of Runx2 & Transglutaminase 2?
Dr. Beatrix Dienes lab Front Physiol 2022
https://www.frontiersin.org/articles/10.3389/fphys.2022.1037230/full
Cardiovascular biologist, Dr. J. Geoffrey Pickering lab, Robarts Research Institute, Western University, London, Ontario, Canada
https://scholar.google.ca/citations?hl=en&user=IbrDx00AAAAJ&view_op
Joined Nov 2022
