Hao Yin @HaoYin@qoto.org

3D hydrogel #BloodBrainBarrier model (EC+SMC+Astrocyte, ɸ 600 µm)
+Defined, zonated flow profiles

Transcriptomics of #EndothelialCell with Disturbed vs Laminar flow for 24 h->
#Lumican in #VascularPermability

Dr. Peter Galie lab Fluids Barriers CNS 2022
https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-022-00389-x

#Pericyte internalizes-cleaves-degrades α-Synuclein aggregates, even in co-culture with "professional" Microglia😎 (relative efficiency?🧐)

How α-Syn fibril uptake impact pericyte metabolism & functions?
#ParkinsonsDisease

Dr. Birger Victor Dieriks & Mike Dragunow labs @PLOSONE 2022
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0277658

#Microglia jointly degrade fibrillar α-Synuclein cargo by distribution through #TunnelingNanoTube

#TunnelingNanoTube-mediated social behaviors of Microglia

Microglia take α-Synuclein from & send Mitochondria to overloaded neighbors

Dr, Michael Heneka lab @cellcellpress@twtr.plus2021
https://www.cell.com/cell/fulltext/S0092-8674(21)01054-0

My continued biomedical education

Multimodality imaging of large-vessel #Vasculitis

#GiantCellArteritis #TakayasuArteritis

Aorta/Coronary artery/Pulmonary artery

Echo
CT
MRI
18F-FDG PET

Dr. Jason Tarkin & Deepa Gopalan @Heart_BMJ 2022 @Jmtarkin
https://heart.bmj.com/content/early/2022/11/29/heartjnl-2022-321113

An impressive panorama of Trimethylamine N-oxide/TMAO on👤aortic #EndothelialCell
⏫Traits of #TrainedImmunity/Inflammation
Metabolic reprogramming
Kinome & transcriptional network

Intriguing role of PERK & mitoROS

In addition to Liver, Aorta (#SmoothMuscleCell/#Pericyte/Fibroblast/Mɸ?) may🤔 also oxidize #GutMicrobiota-derived trimethylamine/TMA into Trimethylamine N-oxide/TMAO

What is the local/Aortic vs circulating TMAO level?🧐

Wait for🐭tissue-specific KO of FMO3 (Oxidizing TMA)😆

Dr. Xiaofeng Yang lab JCI insight 2022
https://insight.jci.org/articles/view/158183

#Sirt6 #VascularInflammation

MRTF-A as a deacetylation target of Sirt6 in #EndothelialCell

Deactylated MRTF-A stays in cytoplasm, failing to mediate oxLDL-induced EC ICAM1

An OxLDL-DNMT1 cacade epigenetically silences Sirt6 expression

Dr. Qianyun Wang, Tianfa Li & Qianwen Zhao labs Cell Death Discov 2022
https://www.nature.com/articles/s41420-022-00903-y

#SIRT6 N308K/A313S, a #Centenarian variant
⏬deacetylase/deacylase
⏫mono-ADP-ribosylase
⏬LINE1 retrotransposition
⏫DNA repair
⏫tumor killing

Lamin A is mADP-ribosylated by Sirt6!

Love to explore its benefit over blood vessels!

Dr. Andrei Seluanov, Yousin Suh, Vera Gorbunova labs EMBO J 2022
https://www.embopress.org/doi/full/10.15252/embj.2021110393

Multi-agent-based computational modeling of #Lumenogenesis from a single proliferating cell

High Matrix density & dynamic viscosity->
Formation of multiluminal structure instead of a single cyst lumen

This is a very easy-to-read computational modeling paper (minimal math requirement😁)
Cell cycle/polarity/secretion
Cell-cell (attractive+repulsive) + cell-fluid interaction (repulsive) + cell-matrix interactions
Geometry & Biomechanics (but not biochemical property of matrix)

Dr. José Manuel García-Aznar lab Eng Comput 2022
https://link.springer.com/article/10.1007/s00366-022-01654-1

K63 #Ubiquitination of #YAP by SKP2 #E3Ligase⏫its nuclear import🤠

Synergized with mTORC1-PERK-ATF4 axis in renal #Cystogenesis🐭#JuvenileCysticKidney #Ciliopathy

A #CellCycle-specific regulation of YAP?😆

Dr. Andrew Karaplis lab JCI 2022
https://www.jci.org/articles/view/153943

#MatrixStiffness (4 vs 11 kPa) of GelMA #Hydrogel Substrate regulates👤Lung Fibroblast

Proliferation
Cytoskeleton
Matrix deposition (Decorin/Collagen/Microfibril)
Secretome (IL6)

Fig 4↔️6?🧐

Dr. Janette Burgess lab Front Pharmacol 2022
https://www.frontiersin.org/articles/10.3389/fphar.2022.989169/full

Induced #EndothelialCell KLF4 KO in adult🐭->
Lung Inflammation+Fibrosis within 1 month😎

KLF4-KO EC has⏫KLF2 & #EndMT simultaneously!
Perturbed shear-sensing?
Link b/w⏫KLF2 & #EndMT?
EC secretome? So interesteing😆

Dr. Kishore Wary lab @FrontCellDevBio 2022 @KishoreWary
https://www.frontiersin.org/articles/10.3389/fcell.2022.1003028/full

A potential FGF9-TCF7L2 (#Wnt)-HAS2/#HyaluronicAcid cascade in #Palatogenesis #CleftPalate

GeneMANIA, a useful resource to explore gene relationships
http://www.genemania.org

Dr. Zhenqi Chen lab @Biomol_MDPI 2022
https://www.mdpi.com/2218-273X/12/11/1639/htm

Itga8-CreERT2, a novel tamoxifen-inducible #SmoothMuscleCell-specific Cre🐭

Selective for Vascular over Visceral (GI/Airway...) SMC🤠

Critical comparison (+Aorta transcriptomes) vs Myh11-CreERT2

Dr. Qing Lyu & Joseph Miano labs Nature Cardiovascular Research 2022
https://www.nature.com/articles/s44161-022-00162-1

Normal & abnormal development of the aortic valve & ascending aortic wall: a comprehensive overview of the Embryology & Pathology of the #BicuspidAorticValve

#AortaEd

Dedication to Dr. Adriana Gittenberger-De Groot

Dr. Nimrat Grewal & colleagues Ann Cardiothorac Surg 2022
https://www.annalscts.com/article/view/16943/html

Chromatin landscape dynamics of #SmoothMuscleCell in #Atherosclerosis🐭

Integrated scATACseq+scRNAseq+lineage tracing analysis reveals a protective role of ATF3 in atherogenic #Complement 3+ SMC

Dr. Ying Wang & Nicholas Leeper lab #CardiovascRes 2022
https://academic.oup.com/cardiovascres/article/118/13/2792/6438000

Decoupling #TGFβ1 with phospho-SMAD2 (S457) & phospho-SMAD3 (S423/425) in ascending #AorticAneurysm with #BicuspidAorticValve or #UnicuspidAorticValve

How about TGFβ2/3?

As a comparison, in #TricuspidAorticValve aorta, diameter and elastin break correlate well with increased TGFβ1 level or p-SMAD2/3 levels

Dr. Brittany Balint & Hans-Joachim Schäfers lab, Sci Rep 2022
https://www.nature.com/articles/s41598-022-19335-w

Airway #SmoothMuscleCell forms Collagen III microniche⏫🫁#Metastasis of #BladderCancer
via DDR1-HSP90-Stat3 axis

In primary #MuscleInvasiveBladderCarcinoma Collagen I-DDR1 axis⏫tumor invasiveness

Dr. Keith Syson Chan lab Nature Comms 2019
https://www.nature.com/articles/s41467-019-09878-4

AORTA score, a predictive model trained +common features (age/sex/BP...) in >30k #UKBiobank👤 explains 28-33% #AscendingAorticDiameter
Dr. James Pirruccello
Dr. Mark Lindsay @MarkELindsay Dr. Patrick Ellinor
JAMA 2022
https://jamanetwork.com/journals/jama/article-abstract/2798406

Enjoy the great🧵 by Dr. James Pirruccello
https://twitter.com/jpirruccello/status/1592596235751665666

Abdominal Aortic Calcification in #UKBiobank

38264👤
#MachineLearning to measure AAC

-Physio/Biochem predictors
-Cardiovascular outcomes
-Genetics TWIST1/HDAC9, FGF9, NAV1, APOE

Dr. Madeleine Cule & Eugene Melamud lab Frontiers Cardiovascular Medicine 2022
https://www.frontiersin.org/articles/10.3389/fcvm.2022.1003246/full

Evidence on #EndMT in human #MultipleSclerosis

Inflammation-induced endothelial to mesenchymal transition promotes brain endothelial cell dysfunction & occurs during multiple sclerosis pathophysiology

Dr. Helga de Vries lab Cell Death Dis 2019
https://www.nature.com/articles/s41419-018-1294-2